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Extracts from interferon-treated, not virus infected EAT cells differ in several biochemical characteristics from extracts of untreated cells. Some of these differences are manifested only if the extracts are supplemented with ds RNA and ATP. Thus, in the extracts from interferon-treated cells these supplements activate a protein kinase and an endonuclease activity as well as an inhibitor of the translation of messenger RNA. The effect of the same supplements in extracts of untreated cells is much less pronounced. Other differences between the two types of extracts do not seem to depend on the addition of ds RNA and ATP. These include an impairment of mRNA cap methylation and an inhibition of peptide chain elongation that can be overcome by the addition of tRNA. The treatment of human (HeLa S3) cells with human interferon is manifested in the cell extract similarly to the treatment of EAT cells with mouse interferon. Studies are underway to isolate and characterize the ds RNA activated enzymes and the inhibitors and to establish how the presence of these in extracts from interferon-treated cells can account for the impairment of virus replication by interferon.
G C Sen, and
R Desrosiers, and
L Ratner, and
S Shaila, and
G E Brown, and
B Lebleu, and
E Slattery, and
M Kawakita, and
B Cabrer, and
H Taira, and
P Lengyel
July 1975,
Biochemical and biophysical research communications,
G C Sen, and
R Desrosiers, and
L Ratner, and
S Shaila, and
G E Brown, and
B Lebleu, and
E Slattery, and
M Kawakita, and
B Cabrer, and
H Taira, and
P Lengyel
November 1976,
Journal of molecular biology,
G C Sen, and
R Desrosiers, and
L Ratner, and
S Shaila, and
G E Brown, and
B Lebleu, and
E Slattery, and
M Kawakita, and
B Cabrer, and
H Taira, and
P Lengyel
May 1972,
Proceedings of the National Academy of Sciences of the United States of America,
G C Sen, and
R Desrosiers, and
L Ratner, and
S Shaila, and
G E Brown, and
B Lebleu, and
E Slattery, and
M Kawakita, and
B Cabrer, and
H Taira, and
P Lengyel
November 1977,
Virology,
G C Sen, and
R Desrosiers, and
L Ratner, and
S Shaila, and
G E Brown, and
B Lebleu, and
E Slattery, and
M Kawakita, and
B Cabrer, and
H Taira, and
P Lengyel
April 1974,
Biochemical and biophysical research communications,
G C Sen, and
R Desrosiers, and
L Ratner, and
S Shaila, and
G E Brown, and
B Lebleu, and
E Slattery, and
M Kawakita, and
B Cabrer, and
H Taira, and
P Lengyel
January 1979,
Acta biologica et medica Germanica,
G C Sen, and
R Desrosiers, and
L Ratner, and
S Shaila, and
G E Brown, and
B Lebleu, and
E Slattery, and
M Kawakita, and
B Cabrer, and
H Taira, and
P Lengyel
August 1981,
The Journal of biological chemistry,
G C Sen, and
R Desrosiers, and
L Ratner, and
S Shaila, and
G E Brown, and
B Lebleu, and
E Slattery, and
M Kawakita, and
B Cabrer, and
H Taira, and
P Lengyel
January 1974,
Journal of virology,
G C Sen, and
R Desrosiers, and
L Ratner, and
S Shaila, and
G E Brown, and
B Lebleu, and
E Slattery, and
M Kawakita, and
B Cabrer, and
H Taira, and
P Lengyel
September 1977,
Acta virologica,
G C Sen, and
R Desrosiers, and
L Ratner, and
S Shaila, and
G E Brown, and
B Lebleu, and
E Slattery, and
M Kawakita, and
B Cabrer, and
H Taira, and
P Lengyel
May 1977,
Proceedings of the National Academy of Sciences of the United States of America,
