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The complete structure of oligosaccharides from locust lipophorin was studied. The asparagine-linked oligosaccharides were first liberated from the protein moiety of lipophorin by digestion with almond glycopeptidase (N-oligosaccharide glycopeptidase, EC 3.5.1.52). Two major oligosaccharides (E and F), separated by subsequent thin-layer chromatography, were analyzed by methylation analysis and 1H-NMR. Based on the experimental data, the whole structure of oligosaccharide E was identified as Man alpha 1----2Man alpha 1----6(Man alpha 1----2Man alpha 1----3) Man alpha 1----6(Man alpha 1----2Man alpha 1----2Man alpha 1----3)Man beta 1----4GlcNAc beta 1----4GlcNAc. The data also revealed that oligosaccharide F is identical with oligosaccharide E in the structure, except for one glucose residue that is linked to the nonreducing terminal Man alpha 1----2 residue.
E Nagao, and
H Chino
January 1982,
Methods in enzymology,
E Nagao, and
H Chino
December 1985,
Proceedings of the National Academy of Sciences of the United States of America,
E Nagao, and
H Chino
September 1988,
Biochemistry,
E Nagao, and
H Chino
January 1985,
Annual review of biochemistry,
E Nagao, and
H Chino
January 1982,
Methods in enzymology,
E Nagao, and
H Chino
January 1986,
The Biochemical journal,
E Nagao, and
H Chino
January 1989,
Pharmacology & therapeutics,
E Nagao, and
H Chino
May 1987,
Analytical biochemistry,
E Nagao, and
H Chino
February 1989,
Biochemical Society transactions,
E Nagao, and
H Chino
January 2003,
Methods in enzymology,
