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A radioimmunoassay for thromboxane B2 (TXB2) in unextracted horse plasma was evaluated. Sensitivity of the assay was 14.0 (SD 5.6) pg ml-1 of plasma. Interassay and intra-assay variation were 21.3 per cent and 4.3 per cent, respectively. The percentage of tracer bound in unextracted plasma in the absence of TXB2 was often higher than that in buffer. Therefore standard curves were obtained using standards diluted in plasma from horses treated with aspirin or in charcoal treated TXB2-free plasma. Standard curves determined in plasma and buffer were parallel. This assay was used to determine the half-life of exogenous TXB2 in horses. The mean value of 20.7 minutes (n = 3) is similar to that determined in other species. Storage of plasma samples at -20 degrees C for four months was found to alter the TXB2 levels in an unpredictable manner. Mean recovery was 102.4 per cent (SD 44.1). Effect of variability in sample collection and handling was assessed, but no consistent source of artifactual generation of TXB2 was found.
H Cambridge, and
J A Reynoldson, and
J D Dunsmore, and
B J Hilbert
August 1981,
Clinical chemistry,
H Cambridge, and
J A Reynoldson, and
J D Dunsmore, and
B J Hilbert
September 1988,
Thrombosis research,
H Cambridge, and
J A Reynoldson, and
J D Dunsmore, and
B J Hilbert
April 1986,
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae,
H Cambridge, and
J A Reynoldson, and
J D Dunsmore, and
B J Hilbert
May 1982,
Prostaglandins, leukotrienes, and medicine,
H Cambridge, and
J A Reynoldson, and
J D Dunsmore, and
B J Hilbert
January 1982,
Methods in enzymology,
H Cambridge, and
J A Reynoldson, and
J D Dunsmore, and
B J Hilbert
September 1977,
Analytical biochemistry,
H Cambridge, and
J A Reynoldson, and
J D Dunsmore, and
B J Hilbert
January 1980,
Advances in prostaglandin and thromboxane research,
H Cambridge, and
J A Reynoldson, and
J D Dunsmore, and
B J Hilbert
October 1980,
Prostaglandins,
H Cambridge, and
J A Reynoldson, and
J D Dunsmore, and
B J Hilbert
July 1978,
Analytical biochemistry,
H Cambridge, and
J A Reynoldson, and
J D Dunsmore, and
B J Hilbert
January 1985,
Advances in prostaglandin, thromboxane, and leukotriene research,
