Biomaterial Database

Immunohistochemical and Electron Microscopy Evolution in the Diagnosis of Lung Cancer in Iraq. 2022

S Saleem Abd Alkreem Alsaqi

Department of Anatomy, College of Medicine, University of Babylon, Babylon, Iraq.

Lung cancer is considered the most commonly diagnosed cancer that kills over 1.76 million people yearly around the world. This study aimed to investigate how electron microscopy (EM) and immunohistochemistry (IHC) worked together. In the current study, a total of 50 tissue blocks were used which were embedded in paraffin wax from 20 healthy controls and 30 lung cancer patients (19 males and 11 females) to conduct a histopathological examination, which included hematoxylin and eosin stain, IHC detection regarding the protein expression in P53, and EM for lung cancer diagnosis. The findings indicated that the mean age of the patients was 51.84±15.70 years, and there was no correlation between the age of patients and the disease stage. P53 expression was measured using an immunohistochemical technique, while the positive rate showed a highly significant difference between healthy controls and patients (PP≤0.05). The association of P53 expression with age and gender was not significant, although it showed a significant correlation with stage and grade. Furthermore, a correlation was found between P53 IHC and EM (P≤0.05). In conclusion, the detection of an immunohistochemical method of lung cancer patients has been (gold standard) useful in detecting the subsets of patients in addition to a good method of EM in the diagnosis of lung cancer patients.

UI	MeSH Term	Description	Entries
D007150	Immunohistochemistry	Histochemical localization of immunoreactive substances using labeled antibodies as reagents.	Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D007493	Iraq	A country bordering the Persian Gulf, between Iran and Kuwait. The capital is Baghdad.	Republic of Iraq
D008175	Lung Neoplasms	Tumors or cancer of the LUNG.	Cancer of Lung,Lung Cancer,Pulmonary Cancer,Pulmonary Neoplasms,Cancer of the Lung,Neoplasms, Lung,Neoplasms, Pulmonary,Cancer, Lung,Cancer, Pulmonary,Cancers, Lung,Cancers, Pulmonary,Lung Cancers,Lung Neoplasm,Neoplasm, Lung,Neoplasm, Pulmonary,Pulmonary Cancers,Pulmonary Neoplasm
D008297	Male		Males
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D016159	Tumor Suppressor Protein p53	Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.	p53 Tumor Suppressor Protein,Cellular Tumor Antigen p53,Oncoprotein p53,TP53 Protein,TRP53 Protein,p53 Antigen,pp53 Phosphoprotein,Phosphoprotein, pp53