Biomaterial Database

Immunoproteome profiling of Listeria monocytogenes under mild acid and salt stress conditions. 2022

Federica D'Onofrio, and Pierina Visciano, and Ivanka Krasteva, and Marina Torresi, and Manuela Tittarelli, and Francesco Pomilio, and Luigi Iannetti, and Tiziana Di Febo, and Antonello Paparella, and Maria Schirone, and Mirella Luciani

Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy.

Listeria monocytogenes is one of the main foodborne pathogens worldwide. Although its response to stress conditions has been extensively studied, it is still present in the food processing environments and is a concern for consumers. To investigate how this microorganism adapts its proteome in mild stress conditions, a combined proteomics and bioinformatics approach was used to characterize the immunogenic protein profile of a sequence type 7 (ST7) strain that caused severe listeriosis outbreaks in central Italy. Extracted proteins were analyzed by immunoblotting using positive sera against L. monocytogenes and nLC-ESI-MS/MS, and all data were examined by five software to predict subcellular localization (SCL). A total of 226 proteins were extracted from the bands of interest, 58 of which were classified as potential immunogenic antigens. Compared to control cells grown under optimal conditions, six proteins, some of which under-described, were expressed under mild acid and salt stress conditions and/or at 12°C. In particular, adaptation and shaping of the proteome mainly involved cell motility at 12°C without acid and salt stress, whereas the combination of the same temperature with mild acid and salt stress induced a response concerning carbohydrate metabolism, oxidative stress, and DNA repair. Raw data are available via ProteomeXchange with identifier PXD033519.

UI	MeSH Term	Description	Entries
D008089	Listeria monocytogenes	A species of gram-positive, rod-shaped bacteria widely distributed in nature. It has been isolated from sewage, soil, silage, and from feces of healthy animals and man. Infection with this bacterium leads to encephalitis, meningitis, endocarditis, and abortion.
D000077323	Salt Stress	The condition that results from ion toxicity due to ion imbalances (e.g., excessive Na+ and deficiency in Mg++).	Salinity Stress,Salinity Stress Reaction,Salinity Stress Reponse,Salt Stress Reaction,Salt Stress Response,Reaction, Salinity Stress,Reaction, Salt Stress,Reponse, Salinity Stress,Response, Salt Stress,Salinity Stress Reactions,Salinity Stress Reponses,Salinity Stresses,Salt Stress Reactions,Salt Stress Responses,Salt Stresses,Stress Reaction, Salinity,Stress Reaction, Salt,Stress Reponse, Salinity,Stress Response, Salt,Stress, Salinity,Stress, Salt
D000143	Acids	Chemical compounds which yield hydrogen ions or protons when dissolved in water, whose hydrogen can be replaced by metals or basic radicals, or which react with bases to form salts and water (neutralization). An extension of the term includes substances dissolved in media other than water. (Grant & Hackh's Chemical Dictionary, 5th ed)	Acid
D053719	Tandem Mass Spectrometry	A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.	Mass Spectrometry-Mass Spectrometry,Mass Spectrometry Mass Spectrometry,Mass Spectrometry, Tandem
D020543	Proteome	The protein complement of an organism coded for by its genome.	Proteomes