Dual-mode colorimetric determination of As(III) based on negatively-charged aptamer-mediated aggregation of positively-charged AuNPs. 2022

Dongwei Zhang, and Shaohua Chu, and Lumei Wang, and Xuejia Zhan, and Pei Zhou, and Dan Zhang
School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai, 200240, China; Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, 800 Dongchuan Rd., Shanghai, 200240, China; Shanghai Yangtze River Delta Eco-environmental Change and Management Observation and Research Station, Ministry of Science and Technology, China.

The aggregation and redispersion of positively-charged AuNPs ((+)AuNPs) modified with cysteamine (CS) could be regulated by the negatively charged As(III)-specific aptamer (As(III)-apt). In general, (+)AuNPs aggregated with increasing inducer concentration. However, in the present study, it was found that (+)AuNPs re-dispersed after a certain high concentration of As(III)-apt was reached. By optimizing the concentration of As(III)-apt that resulted in the aggregation and redispersion behavior of (+)AuNPs, a dual-mode colorimetric aptasensor for As(III) determination was established. It was not only able to quantify As(III) sensitively over a ranges of As(III) concentrations, but also to selectively differentiate As(III) and eliminate false results from other control ions by dual-mode.

UI MeSH Term Description Entries
D003124 Colorimetry Any technique by which an unknown color is evaluated in terms of standard colors. The technique may be visual, photoelectric, or indirect by means of spectrophotometry. It is used in chemistry and physics. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D006046 Gold A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
D015374 Biosensing Techniques Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal. Bioprobes,Biosensors,Electrodes, Enzyme,Biosensing Technics,Bioprobe,Biosensing Technic,Biosensing Technique,Biosensor,Electrode, Enzyme,Enzyme Electrode,Enzyme Electrodes,Technic, Biosensing,Technics, Biosensing,Technique, Biosensing,Techniques, Biosensing
D052157 Aptamers, Nucleotide Nucleotide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity. DNA Aptamer,DNA Aptamers,RNA Aptamers,Rna Aptamer,Nucleotide Aptamers,Oligonucleotide Ligands, DNA,Oligonucleotide Ligands, RNA,Aptamer, DNA,Aptamer, Rna,Aptamers, DNA,Aptamers, RNA,DNA Oligonucleotide Ligands,RNA Oligonucleotide Ligands
D053768 Metal Nanoparticles Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver. Metal Nanocrystals,Metallic Nanocrystals,Metallic Nanoparticles,Metal Nanocrystal,Metal Nanoparticle,Metallic Nanocrystal,Metallic Nanoparticle,Nanocrystal, Metal,Nanocrystal, Metallic,Nanocrystals, Metal,Nanocrystals, Metallic,Nanoparticle, Metal,Nanoparticle, Metallic,Nanoparticles, Metal,Nanoparticles, Metallic

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