A linear method for determining specific activity of tracers in radioimmunoassays. 1987

C S Chiang

I describe a rapid and objective method for graphically determining specific activity of radioactive tracers by self-displacement. Two sets of results are plotted on the same graph: total/bound counts vs concentration of unlabeled analyte (for the standard curve results) or quantity of radioactivity (for tracer self-displacement results). Because these plots are linear, not only are the difficulties of curve fitting and the need for numerous data points avoided, but also how well the data fit the lines can be assessed by calculating the standard deviation of the slope of the lines. This method minimizes uncertainty between data points, allows easier interpolation, and often yields more precise results than do previously published procedures.

UI MeSH Term Description Entries
D007457 Iodine Radioisotopes Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes. Radioisotopes, Iodine
D011851 Radioactivity The spontaneous transformation of a nuclide into one or more different nuclides, accompanied by either the emission of particles from the nucleus, nuclear capture or ejection of orbital electrons, or fission. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Nuclear Decay,Radioactive Disintegration,Decay, Nuclear,Disintegration, Radioactive,Disintegrations, Radioactive,Radioactive Disintegrations,Radioactivities
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D012044 Regression Analysis Procedures for finding the mathematical function which best describes the relationship between a dependent variable and one or more independent variables. In linear regression (see LINEAR MODELS) the relationship is constrained to be a straight line and LEAST-SQUARES ANALYSIS is used to determine the best fit. In logistic regression (see LOGISTIC MODELS) the dependent variable is qualitative rather than continuously variable and LIKELIHOOD FUNCTIONS are used to find the best relationship. In multiple regression, the dependent variable is considered to depend on more than a single independent variable. Regression Diagnostics,Statistical Regression,Analysis, Regression,Analyses, Regression,Diagnostics, Regression,Regression Analyses,Regression, Statistical,Regressions, Statistical,Statistical Regressions
D006207 Half-Life The time it takes for a substance (drug, radioactive nuclide, or other) to lose half of its pharmacologic, physiologic, or radiologic activity. Halflife,Half Life,Half-Lifes,Halflifes
D014285 Triiodothyronine, Reverse A metabolite of THYROXINE, formed by the peripheral enzymatic monodeiodination of T4 at the 5 position of the inner ring of the iodothyronine nucleus. 3,3',5'-Triiodothyronine,Reverse T3 Thyroid hormone,Reverse Triiodothyronine,3,3,5 Triiodothyronine,3,3,5-Triiodothyronine

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