Biomaterial Database

Mannose-receptor ligands stimulate secretion of lysosomal enzymes from rabbit alveolar macrophages. 1987

Y Ohsumi, and Y C Lee

Neoglycoproteins (Lee, Y.C., Stowell, C.P., and Krantz, M.J. (1976) Biochemistry 15, 3956-3963) and monosaccharides which interact with the Man/Fuc receptor (Stah, P.D., Rodman, J.S., Miller, M.J., and Schlesinger, P.H. (1978) Proc. Natl. Acad. Sci. U.S.A. 75, 1399-1403) stimulate lysosomal enzyme secretion in rabbit alveolar macrophages in a dose- and time-dependent fashion. Man43-bovine serum albumin (BSA), L-Fuc30-BSA, and Man96-poly-D-lysine (Hoppe, C.A., and Lee, Y.C. (1984) Biochemistry 23, 1723-1730) were potent stimulators of lysosomal enzyme secretion. Mannose, L-fucose (Fuc), N-acetylmannosamine and N-acetylglucosamine were also effective, but much higher concentrations (above 10 mM) were required to elicit the same effects as the corresponding neoglycoproteins. After a 1.5-h incubation of the cells with Man43-BSA in the presence of 10 mM EDTA, the stimulation of lysosomal enzyme secretion was reduced by 73%. These results indicate that the Man/L-Fuc receptor participates in the process of lysosomal enzyme secretion. Addition of cycloheximide did not affect the stimulation by Man43-BSA, indicating that shunting of newly synthesized enzymes is not involved in the observed phenomenon. The temporary binding of ligands to the cell surface Man/Fuc receptor at 4 degrees C also did not show the stimulation effect. Secretion of Man211-poly-D-lysine preloaded in secondary lysosome was stimulated by Man43-BSA, suggesting that secondary lysosome is one of the pools from which lysosomal enzymes are secreted. However, neither storage of an undegradable ligand in lysosomes nor the degradation of a degradable ligand was an absolute requirement for stimulation of enzyme secretion, because both Man96-poly-D-lysine (nondegradable ligand) and Man43-BSA (degradable ligand) can stimulate to a similar extent.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008024	Ligands	A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)	Ligand
D008247	Lysosomes	A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured or undergoes MEMBRANE FUSION. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed).	Autolysosome,Autolysosomes,Lysosome
D008264	Macrophages	The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)	Bone Marrow-Derived Macrophages,Monocyte-Derived Macrophages,Macrophage,Macrophages, Monocyte-Derived,Bone Marrow Derived Macrophages,Bone Marrow-Derived Macrophage,Macrophage, Bone Marrow-Derived,Macrophage, Monocyte-Derived,Macrophages, Bone Marrow-Derived,Macrophages, Monocyte Derived,Monocyte Derived Macrophages,Monocyte-Derived Macrophage
D008358	Mannose	A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)	D-Mannose,Mannopyranose,Mannopyranoside,D Mannose
D011107	Polylysine	A peptide which is a homopolymer of lysine.	Epsilon-Polylysine,Poly-(Alpha-L-Lysine),Epsilon Polylysine
D011817	Rabbits	A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes.	Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D011956	Receptors, Cell Surface	Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.	Cell Surface Receptor,Cell Surface Receptors,Hormone Receptors, Cell Surface,Receptors, Endogenous Substances,Cell Surface Hormone Receptors,Endogenous Substances Receptors,Receptor, Cell Surface,Surface Receptor, Cell
D011971	Receptors, Immunologic	Cell surface molecules on cells of the immune system that specifically bind surface molecules or messenger molecules and trigger changes in the behavior of cells. Although these receptors were first identified in the immune system, many have important functions elsewhere.	Immunologic Receptors,Immunologic Receptor,Immunological Receptors,Receptor, Immunologic,Receptors, Immunological
D006023	Glycoproteins	Conjugated protein-carbohydrate compounds including MUCINS; mucoid, and AMYLOID glycoproteins.	C-Glycosylated Proteins,Glycosylated Protein,Glycosylated Proteins,N-Glycosylated Proteins,O-Glycosylated Proteins,Glycoprotein,Neoglycoproteins,Protein, Glycosylated,Proteins, C-Glycosylated,Proteins, Glycosylated,Proteins, N-Glycosylated,Proteins, O-Glycosylated