Biomaterial Database

Mobile nylon bag for estimating intestinal availability of rumen undegradable protein. 1987

G de Boer, and J J Murphy, and J J Kennelly

The objective was to develop a method for estimating intestinal availability of rumen undegradable protein. One-gram samples of six feedstuffs were placed into small (3.5 X 5.5 cm) nylon bags (pore size 48 micron). Bags were heat sealed and ruminally incubated in a polyester mesh (lingerie) bag in quadruplicate for each of six time periods (0, 2, 4, 8, 12, 24 h). Two bags from each rumen incubation time were inserted into the small intestine via a duodenal cannula at the rate of one bag per 45 min. Bags were mechanically washed either upon removal from the rumen--for determination of rumen degradability, or when recovered from the feces--for estimation of intestinal availability. Dried bags plus contents were digested intact for nitrogen analysis. Rumen degradable protein and apparent intestinal availability of undegradable protein (based on 8 h of rumen incubation) for corn gluten meal, fish meal, meat and bone meal, soybean meal, canola meal, and alfalfa hay, respectively, were 11.8, 95.0; 28.5, 82.8; 46.7, 73.0; 55.1, 99.3; 69.7, 79.2; and 68.8, 71.2. Results support the use of this technique as a rapid method for estimating both ruminal degradability, and intestinal availability of rumen undegradable protein.

UI	MeSH Term	Description	Entries
D007422	Intestines	The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE.	Intestine
D011506	Proteins	Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.	Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D004063	Digestion	The process of breakdown of food for metabolism and use by the body.
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012417	Rumen	The first stomach of ruminants. It lies on the left side of the body, occupying the whole of the left side of the abdomen and even stretching across the median plane of the body to the right side. It is capacious, divided into an upper and a lower sac, each of which has a blind sac at its posterior extremity. The rumen is lined by mucous membrane containing no digestive glands, but mucus-secreting glands are present in large numbers. Coarse, partially chewed food is stored and churned in the rumen until the animal finds circumstances convenient for rumination. When this occurs, little balls of food are regurgitated through the esophagus into the mouth, and are subjected to a second more thorough mastication, swallowed, and passed on into other parts of the compound stomach. (From Black's Veterinary Dictionary, 17th ed)	Rumens