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A rapid, specific, and sensitive (requiring only 20 fmole of antigen equivalent to 0.007 microliter of serum) radioimmunoassay (RIA) was developed for the measurement of guinea pig corticosteroid-binding globulin (CBG). CBG was purified to homogeneity from guinea pig serum by affinity chromatography and used for immunization, as the standard and as the radiolabeled trace in the RIA. The antiserum to CBG was raised in rabbits. It was judged specific by immunoelectrophoresis and by comparison of RIA values with steroid-binding assay profiles obtained on serum separated on the basis of size and ion-exchange properties. The results of the radioimmunoassays agree with those of a steroid-binding assay run on identical samples. The sensitivity of the assay allows detection of CBG in serial serum samples, other biologic fluids such as milk, and cell culture supernatants.
B R Hsu, and
E A Kato, and
W J Raymoure, and
R W Kuhn
March 1983,
Endocrinology,
B R Hsu, and
E A Kato, and
W J Raymoure, and
R W Kuhn
July 1983,
Clinical chemistry,
B R Hsu, and
E A Kato, and
W J Raymoure, and
R W Kuhn
February 1985,
The Journal of endocrinology,
B R Hsu, and
E A Kato, and
W J Raymoure, and
R W Kuhn
November 1981,
Endocrinology,
B R Hsu, and
E A Kato, and
W J Raymoure, and
R W Kuhn
December 1981,
Journal of steroid biochemistry,
B R Hsu, and
E A Kato, and
W J Raymoure, and
R W Kuhn
June 1979,
Biochemistry,
B R Hsu, and
E A Kato, and
W J Raymoure, and
R W Kuhn
January 1971,
Problemy endokrinologii,
B R Hsu, and
E A Kato, and
W J Raymoure, and
R W Kuhn
December 1982,
Endocrinology,
B R Hsu, and
E A Kato, and
W J Raymoure, and
R W Kuhn
February 1980,
Biochemistry,
B R Hsu, and
E A Kato, and
W J Raymoure, and
R W Kuhn
February 1981,
Clinical endocrinology,
