-transparent.png){kind=logo}
Genetic analysis of a mutant of Escherichia coli resistant to the antibiotic mocimycin is presented. This resistance is due to alterations in both tuf genes coding for the elongation factor Tu. Mocimycin resistance is recessive. Bacteria carryong only one tuf gene from the resistant mutant are still mocimycin sensitive. If the mutant gene is the tufA gene, the seisitive cells can be made resistant through inactivation of the tufB gene by insertion of the bacteriophage milliunits genome. Conditional mocimycin-resistant mutants ban also be isolated when the tufB gene is altered by an amber or a temperature-sensitive mutation. When only the tufB allele from the original mocimycin-resistant mutant is present, inactivation of the wild-type tufA gene fails to give viable mocimycin-resistant progeny. We conclude that the tufA mutant allele codes for a functional mocimycin-resistant EF-Tu, whereas the mutant tufB gene does not code for a functional product.
J A Van de Klundert, and
P H Van der Meide, and
P Van de Putte, and
L Bosch
October 1977,
Proceedings of the National Academy of Sciences of the United States of America,
J A Van de Klundert, and
P H Van der Meide, and
P Van de Putte, and
L Bosch
February 1974,
Proceedings of the National Academy of Sciences of the United States of America,
J A Van de Klundert, and
P H Van der Meide, and
P Van de Putte, and
L Bosch
March 1994,
European journal of biochemistry,
J A Van de Klundert, and
P H Van der Meide, and
P Van de Putte, and
L Bosch
May 1976,
Proceedings of the National Academy of Sciences of the United States of America,
J A Van de Klundert, and
P H Van der Meide, and
P Van de Putte, and
L Bosch
February 1986,
Journal of bacteriology,
J A Van de Klundert, and
P H Van der Meide, and
P Van de Putte, and
L Bosch
December 1975,
Proceedings of the National Academy of Sciences of the United States of America,
J A Van de Klundert, and
P H Van der Meide, and
P Van de Putte, and
L Bosch
August 1995,
Archives of biochemistry and biophysics,
J A Van de Klundert, and
P H Van der Meide, and
P Van de Putte, and
L Bosch
March 1980,
Proceedings of the National Academy of Sciences of the United States of America,
J A Van de Klundert, and
P H Van der Meide, and
P Van de Putte, and
L Bosch
November 1979,
Journal of biochemistry,
J A Van de Klundert, and
P H Van der Meide, and
P Van de Putte, and
L Bosch
January 1991,
Biochimie,
