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A commercial preparation of water-soluble acetylcholinesterase from horse red cells has been purified to a specific activity of 2380 U/mg of protein (a 1660-fold purification) by a twofold affinity chromatography on the known sorbent of Sepharose-p-[NH-(CH2)5-C(O)NH(CH2)5C(O)NH-]-C6H4-N+(CH3)3 X Br- at pH 7.5. A selective elution of the enzyme was carried out from 10 mM of the phosphate buffer solution which contains 0.2% of triton X-100. Subsequent desorption of the enzyme proceeded with 5 mM of phenyltrimethylammonium bromide introduced into the buffer. Such effective preparations of acetylcholinesterase have not been previously produced. Effectiveness of the affinity sorbents considerably depends on the nature of the ligand which is covalent-linked with a Sepharose matrix and on the length of the attachment spacer arm ("insert") between them. A reversible inhibitory effect of certain ligands (tetramethylammonium, phenyltrimethylammonium) and their derivatives on acetylcholinesterase is estimated in comparison.
G P Vlasov, and
V R Glushenkova, and
Iu G Zhukovskiĭ, and
O V Lukashevich, and
K V Novozhilov
January 1996,
Prikladnaia biokhimiia i mikrobiologiia,
G P Vlasov, and
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O V Lukashevich, and
K V Novozhilov
June 1975,
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V R Glushenkova, and
Iu G Zhukovskiĭ, and
O V Lukashevich, and
K V Novozhilov
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G P Vlasov, and
V R Glushenkova, and
Iu G Zhukovskiĭ, and
O V Lukashevich, and
K V Novozhilov
August 1995,
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G P Vlasov, and
V R Glushenkova, and
Iu G Zhukovskiĭ, and
O V Lukashevich, and
K V Novozhilov
September 1978,
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G P Vlasov, and
V R Glushenkova, and
Iu G Zhukovskiĭ, and
O V Lukashevich, and
K V Novozhilov
February 1977,
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G P Vlasov, and
V R Glushenkova, and
Iu G Zhukovskiĭ, and
O V Lukashevich, and
K V Novozhilov
September 2011,
Applied biochemistry and biotechnology,
G P Vlasov, and
V R Glushenkova, and
Iu G Zhukovskiĭ, and
O V Lukashevich, and
K V Novozhilov
February 2002,
The international journal of biochemistry & cell biology,
G P Vlasov, and
V R Glushenkova, and
Iu G Zhukovskiĭ, and
O V Lukashevich, and
K V Novozhilov
January 1982,
Biulleten' eksperimental'noi biologii i meditsiny,
G P Vlasov, and
V R Glushenkova, and
Iu G Zhukovskiĭ, and
O V Lukashevich, and
K V Novozhilov
October 1990,
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