Biomaterial Database

Enhancement of transformation and continuous inhibition of intercellular communication by 1-oleoyl-2-acetyl glycerol in BALB/c 3T3 cells. 1987

U Frixen, and H Yamasaki

Tumour-promoting phorbol esters and diacylglycerols have a variety of biochemical and biological effects in common in a number of cell culture systems; thus, it has been suggested that diacylglycerols are endogenous functional analogues of phorbol esters. In order to examine more directly the possible relevance of these functional analogues in tumour promotion, we have studied the promoting effect of 1-oleoyl-2-acetyl glycerol (OAG) in the two-stage transformation model with BALB/c 3T3 cells, which is designed to simulate in vivo two-stage carcinogenesis. When BALB/c 3T3 cells were treated once with a low dose of 3-methylcholanthrene (MCA), almost no increase in cell transformation occurred. However, repeated application of OAG after MCA treatment enhanced cell transformation. OAG enhanced transformation to a lesser extent than a potent promoter of in vivo and in vitro carcinogenesis, phorbol-12,13-didecanoate. OAG also continuously inhibited junctional intercellular communication when BALB/c 3T3 cells were treated daily at confluence. These results indicate that diacylglycerols mimic phorbol esters in promoting cell transformation and that the enhancement may be related to an inhibitory effect on intercellular communication.

UI	MeSH Term	Description	Entries
D008807	Mice, Inbred BALB C	An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research.	BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D002450	Cell Communication	Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.	Cell Interaction,Cell-to-Cell Interaction,Cell Communications,Cell Interactions,Cell to Cell Interaction,Cell-to-Cell Interactions,Communication, Cell,Communications, Cell,Interaction, Cell,Interaction, Cell-to-Cell,Interactions, Cell,Interactions, Cell-to-Cell
D002471	Cell Transformation, Neoplastic	Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.	Neoplastic Transformation, Cell,Neoplastic Cell Transformation,Transformation, Neoplastic Cell,Tumorigenic Transformation,Cell Neoplastic Transformation,Cell Neoplastic Transformations,Cell Transformations, Neoplastic,Neoplastic Cell Transformations,Neoplastic Transformations, Cell,Transformation, Cell Neoplastic,Transformation, Tumorigenic,Transformations, Cell Neoplastic,Transformations, Neoplastic Cell,Transformations, Tumorigenic,Tumorigenic Transformations
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D004075	Diglycerides	Glycerides composed of two fatty acids esterified to the trihydric alcohol GLYCEROL. There are two possible forms that exist: 1,2-diacylglycerols and 1,3-diacylglycerols.	Diacylglycerol,Diacylglycerols
D005989	Glycerides	GLYCEROL esterified with FATTY ACIDS.	Acylglycerol,Acylglycerols
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus