-transparent.png){kind=logo}
An affinity purification system based on elution of cytosolic epoxide hydrolase from a methoxycitronellyl thiol ligand with 4-azidochalcone oxide was applied to a variety of samples including liver from human, monkey, baboon, rabbit, rat and mouse as well as mammary gland from mouse. Hepatic tissues yielded a major 58 kDa band on SDS-PAGE, but the system had to be modified slightly to remove a 33 kDa band for rat. All of the affinity purified hydrolases showed similar properties with regard to substrate selectivity, pH dependence and mobilities on SDS-PAGE.
Related Publications
M H Silva, and
B D Hammock
April 1983,
Biochemical and biophysical research communications,
M H Silva, and
B D Hammock
September 1988,
European journal of biochemistry,
M H Silva, and
B D Hammock
October 1987,
Archives of biochemistry and biophysics,
M H Silva, and
B D Hammock
June 1989,
The Journal of biological chemistry,
M H Silva, and
B D Hammock
January 1982,
Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry,
M H Silva, and
B D Hammock
November 1980,
Life sciences,
M H Silva, and
B D Hammock
January 1986,
Advances in experimental medicine and biology,
M H Silva, and
B D Hammock
July 1982,
European journal of biochemistry,
M H Silva, and
B D Hammock
November 1982,
Biochemistry,
M H Silva, and
B D Hammock
March 1985,
Proceedings of the National Academy of Sciences of the United States of America,
