Effects of cysteamine supplementation on cryopreserved buffalo bull semen quality parameters. 2022

Ahmed Reda Elkhawagah, and Nicola Antonio Martino, and Mohamed Mahmoud Moustafa Kandiel, and Karima Ghoneimy Mahmoud, and Alessandro Ricci, and Leila Vincenti
Dept. of Theriogenology, Faculty of Veterinary Medicine, Benha University, Egypt.

This work aimed to determine the effect of cysteamine (25, 50, 100 and 200 μM) incorporated during dilution on frozen thawed buffalo semen quality. Semen was collected twice weekly for 7 consecutive weeks from three Egyptian buffalo bulls using an artificial vagina. Semen samples were pooled and extended with a Tris-based extender, cooled, equilibrated and finally frozen in liquid nitrogen. The diluted semen was evaluated for motility, viability, morphology, plasma membrane and DNA integrity, in addition to oxidative stress and in vitro fertilizing capability. The post thaw motility and velocity parameters noticeably increased with different concentrations of cysteamine (mainly 100 μM) during different incubation periods. The post thaw sperm viability and normality significantly (p < 0.05) improved with concentrations of 50 and 100 μM. Plasma membrane integrity substantially increased at all concentrations of cysteamine. Cysteamine reduced alanine aminotransferase (at all concentrations), aspartate aminotransferase (at 25-100 μM), and creatine kinase (at 100 and 200 μM). Cysteamine at a concentration of 100 μM noticeably enhanced the total antioxidant capacity and glutathione peroxidase and decreased nitric oxide production. Cysteamine, at concentrations of 100 and 200 μM, increased the DNA intensity in the comet head (%) and decreased the DNA % in the comet tail. The comet tail length and moment substantially decreased at concentrations of 50-200 μM. Cysteamine did not affect the in vitro fertilizing capability of sperm. In conclusion, cysteamine incorporation (mainly at a concentration of 100 μM) in buffalo semen extender showed varying protective effects on different sperm parameters against cryo-damage; however, it did not affect the in vitro fertilizing capacity of sperm.

UI MeSH Term Description Entries
D008297 Male Males
D009569 Nitric Oxide A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP. Endogenous Nitrate Vasodilator,Mononitrogen Monoxide,Nitric Oxide, Endothelium-Derived,Nitrogen Monoxide,Endothelium-Derived Nitric Oxide,Monoxide, Mononitrogen,Monoxide, Nitrogen,Nitrate Vasodilator, Endogenous,Nitric Oxide, Endothelium Derived,Oxide, Nitric,Vasodilator, Endogenous Nitrate
D009584 Nitrogen An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.
D002020 Buffaloes Ruminants of the family Bovidae consisting of Bubalus arnee and Syncerus caffer. This concept is differentiated from BISON, which refers to Bison bison and Bison bonasus. Bubalus,Syncerus,Water Buffaloes,Buffalo,Water Buffalo,Buffalo, Water
D003402 Creatine Kinase A transferase that catalyzes formation of PHOSPHOCREATINE from ATP + CREATINE. The reaction stores ATP energy as phosphocreatine. Three cytoplasmic ISOENZYMES have been identified in human tissues: the MM type from SKELETAL MUSCLE, the MB type from myocardial tissue and the BB type from nervous tissue as well as a mitochondrial isoenzyme. Macro-creatine kinase refers to creatine kinase complexed with other serum proteins. Creatine Phosphokinase,ADP Phosphocreatine Phosphotransferase,ATP Creatine Phosphotransferase,Macro-Creatine Kinase,Creatine Phosphotransferase, ATP,Kinase, Creatine,Macro Creatine Kinase,Phosphocreatine Phosphotransferase, ADP,Phosphokinase, Creatine,Phosphotransferase, ADP Phosphocreatine,Phosphotransferase, ATP Creatine
D003451 Cryoprotective Agents Substances that provide protection against the harmful effects of freezing temperatures. Cryoprotective Agent,Cryoprotective Effect,Cryoprotective Effects,Agent, Cryoprotective,Agents, Cryoprotective,Effect, Cryoprotective,Effects, Cryoprotective
D003543 Cysteamine A mercaptoethylamine compound that is endogenously derived from the COENZYME A degradative pathway. The fact that cysteamine is readily transported into LYSOSOMES where it reacts with CYSTINE to form cysteine-cysteamine disulfide and CYSTEINE has led to its use in CYSTINE DEPLETING AGENTS for the treatment of CYSTINOSIS. Cysteinamine,Mercaptamine,2-Aminoethanethiol,Becaptan,Cystagon,Cysteamine Bitartrate,Cysteamine Dihydrochloride,Cysteamine Hydrobromide,Cysteamine Hydrochloride,Cysteamine Maleate (1:1),Cysteamine Tartrate,Cysteamine Tartrate (1:1),Cysteamine Tosylate,Cysteamine, 35S-Labeled,Mercamine,Mercaptoethylamine,beta-Mercaptoethylamine,2 Aminoethanethiol,35S-Labeled Cysteamine,Bitartrate, Cysteamine,Cysteamine, 35S Labeled,Dihydrochloride, Cysteamine,Hydrobromide, Cysteamine,Hydrochloride, Cysteamine,Tartrate, Cysteamine,Tosylate, Cysteamine,beta Mercaptoethylamine
D005260 Female Females
D005979 Glutathione Peroxidase An enzyme catalyzing the oxidation of 2 moles of GLUTATHIONE in the presence of HYDROGEN PEROXIDE to yield oxidized glutathione and water. Cytosolic Glutathione Peroxidase,Glutathione Lipoperoxidase,Selenoglutathione Peroxidase,Glutathione Peroxidase, Cytosolic,Lipoperoxidase, Glutathione,Peroxidase, Glutathione,Peroxidase, Selenoglutathione
D000410 Alanine Transaminase An enzyme that catalyzes the conversion of L-alanine and 2-oxoglutarate to pyruvate and L-glutamate. (From Enzyme Nomenclature, 1992) EC 2.6.1.2. Alanine Aminotransferase,Glutamic-Pyruvic Transaminase,SGPT,Alanine-2-Oxoglutarate Aminotransferase,Glutamic-Alanine Transaminase,Alanine 2 Oxoglutarate Aminotransferase,Aminotransferase, Alanine,Aminotransferase, Alanine-2-Oxoglutarate,Glutamic Alanine Transaminase,Glutamic Pyruvic Transaminase,Transaminase, Alanine,Transaminase, Glutamic-Alanine,Transaminase, Glutamic-Pyruvic

Related Publications

Ahmed Reda Elkhawagah, and Nicola Antonio Martino, and Mohamed Mahmoud Moustafa Kandiel, and Karima Ghoneimy Mahmoud, and Alessandro Ricci, and Leila Vincenti
February 2014, Reproduction in domestic animals = Zuchthygiene,
Ahmed Reda Elkhawagah, and Nicola Antonio Martino, and Mohamed Mahmoud Moustafa Kandiel, and Karima Ghoneimy Mahmoud, and Alessandro Ricci, and Leila Vincenti
March 2024, Animal reproduction science,
Ahmed Reda Elkhawagah, and Nicola Antonio Martino, and Mohamed Mahmoud Moustafa Kandiel, and Karima Ghoneimy Mahmoud, and Alessandro Ricci, and Leila Vincenti
January 2021, Reproduction in domestic animals = Zuchthygiene,
Ahmed Reda Elkhawagah, and Nicola Antonio Martino, and Mohamed Mahmoud Moustafa Kandiel, and Karima Ghoneimy Mahmoud, and Alessandro Ricci, and Leila Vincenti
January 2018, Cryo letters,
Ahmed Reda Elkhawagah, and Nicola Antonio Martino, and Mohamed Mahmoud Moustafa Kandiel, and Karima Ghoneimy Mahmoud, and Alessandro Ricci, and Leila Vincenti
February 2017, Animal reproduction science,
Ahmed Reda Elkhawagah, and Nicola Antonio Martino, and Mohamed Mahmoud Moustafa Kandiel, and Karima Ghoneimy Mahmoud, and Alessandro Ricci, and Leila Vincenti
January 2016, Polish journal of veterinary sciences,
Ahmed Reda Elkhawagah, and Nicola Antonio Martino, and Mohamed Mahmoud Moustafa Kandiel, and Karima Ghoneimy Mahmoud, and Alessandro Ricci, and Leila Vincenti
October 2016, Theriogenology,
Ahmed Reda Elkhawagah, and Nicola Antonio Martino, and Mohamed Mahmoud Moustafa Kandiel, and Karima Ghoneimy Mahmoud, and Alessandro Ricci, and Leila Vincenti
June 2009, Reproduction in domestic animals = Zuchthygiene,
Ahmed Reda Elkhawagah, and Nicola Antonio Martino, and Mohamed Mahmoud Moustafa Kandiel, and Karima Ghoneimy Mahmoud, and Alessandro Ricci, and Leila Vincenti
March 2015, Andrologia,
Ahmed Reda Elkhawagah, and Nicola Antonio Martino, and Mohamed Mahmoud Moustafa Kandiel, and Karima Ghoneimy Mahmoud, and Alessandro Ricci, and Leila Vincenti
April 2016, Animal reproduction science,
Copied contents to your clipboard!