End-stage renal disease (ESRD) is characterized by progressive loss of kidney function, which can result in damage to various tissues and organs. Dialysis therapy and kidney transplantation are currently the only therapeutic options available for patients with ESRD. In the case of kidney transplantation, organ shortage and high organ rejection have increased the need for novel treatment modalities. Therefore, organ regeneration employing decellularization technology has emerged as a viable alternative to conventional organ transplantation. In this technology, organs are decellularized using physical, chemical, or biological means to create a natural scaffold and foundation for cell seeding. After in vivo transplantation, this scaffold can be recellularized using stem cells or adult differentiated cells, resulting in a functional organ devoid of immune response. This review focuses on the primary agents used for renal decellularization and the current status of kidney regeneration using decellularization.