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In order to develop multi-residues rapid detection, the bispecific aptamers against malachite green (MG) and leucomalachite green (LMG) were isolated by the capture systematic evolution of ligands by exponential enrichment (Capture-SELEX). After thirteen rounds of selection, the enriched ssDNA pools were sent for high-throughput sequencing. Nine aptamer candidates (A1-A9) were picked out to test their specificity by gold nanoparticles (AuNPs) colorimetric assay. Three aptamers (A2, A3, A5) with good selectivity were truncated to verify their affinity by fluorescence assay. Finally, three truncated aptamers (A2-a, A3-a, A5-a) with bispecificity and high affinity were identified. For LMG, the dissociation constant (Kd) of them were 8.4 ± 0.8 nM, 8.2 ± 1.2 nM, and 13.7 ± 1.4 nM, respectively. For MG, Kd of them were 3.4 ± 0.3 μM, 2.3 ± 0.2 μM, 3.0 ± 0.2 μM. Among them, A3-a is the best. Our work will provide novel probes for the development of multi-residues rapid detection as well as opportunities for multiple target aptamer discovery.
Wenwei Wu, and
Qifeng Sun, and
Tiansong Li, and
Kexin Liu, and
Yan Jiang, and
Yi Wang, and
Yongjie Yang
March 2003,
Chemical research in toxicology,
Wenwei Wu, and
Qifeng Sun, and
Tiansong Li, and
Kexin Liu, and
Yan Jiang, and
Yi Wang, and
Yongjie Yang
October 2016,
Journal of agricultural and food chemistry,
Wenwei Wu, and
Qifeng Sun, and
Tiansong Li, and
Kexin Liu, and
Yan Jiang, and
Yi Wang, and
Yongjie Yang
October 1997,
Applied and environmental microbiology,
Wenwei Wu, and
Qifeng Sun, and
Tiansong Li, and
Kexin Liu, and
Yan Jiang, and
Yi Wang, and
Yongjie Yang
January 1999,
Journal of applied toxicology : JAT,
Wenwei Wu, and
Qifeng Sun, and
Tiansong Li, and
Kexin Liu, and
Yan Jiang, and
Yi Wang, and
Yongjie Yang
July 2008,
Analytical and bioanalytical chemistry,
Wenwei Wu, and
Qifeng Sun, and
Tiansong Li, and
Kexin Liu, and
Yan Jiang, and
Yi Wang, and
Yongjie Yang
July 2004,
Mutation research,
Wenwei Wu, and
Qifeng Sun, and
Tiansong Li, and
Kexin Liu, and
Yan Jiang, and
Yi Wang, and
Yongjie Yang
March 2022,
Foods (Basel, Switzerland),
Wenwei Wu, and
Qifeng Sun, and
Tiansong Li, and
Kexin Liu, and
Yan Jiang, and
Yi Wang, and
Yongjie Yang
August 2006,
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
Wenwei Wu, and
Qifeng Sun, and
Tiansong Li, and
Kexin Liu, and
Yan Jiang, and
Yi Wang, and
Yongjie Yang
April 2023,
Environmental science and pollution research international,
Wenwei Wu, and
Qifeng Sun, and
Tiansong Li, and
Kexin Liu, and
Yan Jiang, and
Yi Wang, and
Yongjie Yang
June 2006,
Journal of agricultural and food chemistry,
