BIOMAT Database Logo BIOMAT Database Logo

Evaluation of three commercial ELISA tests for serological detection of maedi-visna virus using Bayesian latent class analysis. 2022

Anniken Jerre, and Anne Bang Nordstoga, and Katharine Rose Dean, and Ingrid Hunter Holmøy
Norwegian Veterinary Institute, P.O. Box 64, 1431 Ås, Norway. Electronic address: anniken.jerre@vetinst.no.

Early and accurate diagnosis is fundamental for successful surveillance and control of maedi-visna virus (MVV). MVV was detected in Norway in 2019, almost 14 years after the previous outbreak. Genetic analysis indicates persistence of the virus in the sheep population since 2005. The virus was not detected despite continuous serological surveillance. This emphasises the need for improved surveillance, which relies on an understanding of both diagnostic test performance, sampling strategy and the prevalence of the disease. This study therefore aims to evaluate three commercial ELISA tests for MVV antibodies. We conducted a retrospective study using 615 samples from six flocks diagnosed with MVV in 2019. We ran all samples with the following three tests: ID Screen® MVV/CAEV Indirect (IDvet, Grabels, France), IDEXX MVV/CAEV p28 Ab Verification Test (IDEXX Laboratories, Maine, USA) and Elitest MVV/CAEV (Hyphen Biomed, Neuville-sur-Oise, France), hereinafter referred to as ID Screen, IDEXXp28 and Elitest respectively. Without a perfect reference test, we used Bayesian latent class analysis, including conditional dependence between tests, to estimate diagnostic accuracy and true prevalence in the flocks. Using recommended cut-off values, we found that ID Screen and Elitest had significantly higher sensitivity (Se) estimates (99.3 % [97.4-100.0, 95 % Posterior Credible Interval] and 97.4 % [94.1-99.7 %], respectively) than IDEXXp28 (79.5 % [72.3-86.0 %]), while IDEXXp28 and ID Screen had significantly higher specificity (Sp) estimates than Elitest (99.7 % [99.1-100.0], 99.1 % [98.0-99.8 %] and 93.7 % [91.4-95.7 %], respectively). The estimated true prevalence in the six flocks ranged from a median of 0.8-93.5 %. Combining ID Screen and Elitest in serial interpretation showed the highest median Se and Sp (96.7 % [92.0-99.1] and 100.0 % [99.9-100.0], respectively), as well as the highest median positive predictive value (PPV) for the population with the lowest prevalence. Our study supports the use of ID Screen for screening. Further verification with Elitest in serial interpretation will enhance the PPV.

UI MeSH Term Description Entries
D011021 Pneumonia, Progressive Interstitial, of Sheep Chronic respiratory disease caused by the VISNA-MAEDI VIRUS. It was formerly believed to be identical with jaagsiekte (PULMONARY ADENOMATOSIS, OVINE) but is now recognized as a separate entity. Maedi,Progressive Interstitial Pneumonia of Sheep
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D000077272 Latent Class Analysis A statistical algorithm used to analyze clusters of observed variables by constructing categorical unobserved or latent segment based on weighted analysis and the average probabilities. Such latent classes are used to infer variables whose relationships are not directly observed. In biomedical research, it is often used to categorize data that allows the determination of symptom clusters. Latent Class Model,Latent Variable Modeling,Probabilistic Latent Semantic Analysis,Structural Equation Modeling,Latent Class Analyses,Latent Class Models,Latent Variable Modelings,Model, Latent Class,Modeling, Latent Variable,Modeling, Structural Equation,Structural Equation Modelings,Variable Modeling, Latent
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001499 Bayes Theorem A theorem in probability theory named for Thomas Bayes (1702-1761). In epidemiology, it is used to obtain the probability of disease in a group of people with some characteristic on the basis of the overall rate of that disease and of the likelihood of that characteristic in healthy and diseased individuals. The most familiar application is in clinical decision analysis where it is used for estimating the probability of a particular diagnosis given the appearance of some symptoms or test result. Bayesian Analysis,Bayesian Estimation,Bayesian Forecast,Bayesian Method,Bayesian Prediction,Analysis, Bayesian,Bayesian Approach,Approach, Bayesian,Approachs, Bayesian,Bayesian Approachs,Estimation, Bayesian,Forecast, Bayesian,Method, Bayesian,Prediction, Bayesian,Theorem, Bayes
D012189 Retrospective Studies Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons. Retrospective Study,Studies, Retrospective,Study, Retrospective
D012756 Sheep Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS. Ovis,Sheep, Dall,Dall Sheep,Ovis dalli
D012757 Sheep Diseases Diseases of domestic and mountain sheep of the genus Ovis. Ovine Diseases,Disease, Ovine,Disease, Sheep,Diseases, Ovine,Diseases, Sheep,Ovine Disease,Sheep Disease
D014790 Visna-maedi virus A species of LENTIVIRUS, subgenus ovine-caprine lentiviruses (LENTIVIRUSES, OVINE-CAPRINE), that can cause chronic pneumonia (maedi), mastitis, arthritis, and encephalomyelitis (visna) in sheep. Maedi is a progressive pneumonia of sheep which is similar to but not the same as jaagsiekte (PULMONARY ADENOMATOSIS, OVINE). Visna is a demyelinating leukoencephalomyelitis of sheep which is similar to but not the same as SCRAPIE. Maedi Virus,Maedi-Visna Virus,Visna Virus,Maedi Viruses,Maedi Visna Virus,Visna Maedi Virus

Related Publications

Anniken Jerre, and Anne Bang Nordstoga, and Katharine Rose Dean, and Ingrid Hunter Holmøy
Evaluation of three serological tests for diagnosis of Maedi-Visna virus infection using latent class analysis.
February 2007, Veterinary microbiology,
Anniken Jerre, and Anne Bang Nordstoga, and Katharine Rose Dean, and Ingrid Hunter Holmøy
Bayesian Evaluation of Three Serological Tests for Diagnosis of Brucella infections in Dromedary Camels Using Latent Class Models.
November 2022, Preventive veterinary medicine,
Anniken Jerre, and Anne Bang Nordstoga, and Katharine Rose Dean, and Ingrid Hunter Holmøy
Evaluation of three serological tests for brucellosis in naturally infected cattle using latent class analysis.
November 2007, Veterinary microbiology,
Anniken Jerre, and Anne Bang Nordstoga, and Katharine Rose Dean, and Ingrid Hunter Holmøy
An ELISA for the detection of maedi/visna antibody.
June 1979, The Veterinary record,
Anniken Jerre, and Anne Bang Nordstoga, and Katharine Rose Dean, and Ingrid Hunter Holmøy
Comparison of serological tests used in three state veterinary laboratories to identify maedi-visna virus infection.
November 1982, The Veterinary record,
Anniken Jerre, and Anne Bang Nordstoga, and Katharine Rose Dean, and Ingrid Hunter Holmøy
Development of a monoclonal antibody blocking-ELISA for detection of antibodies against Maedi-Visna virus.
August 1999, Journal of virological methods,
Anniken Jerre, and Anne Bang Nordstoga, and Katharine Rose Dean, and Ingrid Hunter Holmøy
Evaluation of three serological tests for diagnosis of bovine brucellosis in smallholder farms in Pakistan by estimating sensitivity and specificity using Bayesian latent class analysis.
January 2018, Preventive veterinary medicine,
Anniken Jerre, and Anne Bang Nordstoga, and Katharine Rose Dean, and Ingrid Hunter Holmøy
An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to maedi-visna virus.
July 1982, Veterinary microbiology,
Anniken Jerre, and Anne Bang Nordstoga, and Katharine Rose Dean, and Ingrid Hunter Holmøy
Modeling the accuracy of a novel PCR and antibody ELISA for African swine fever virus detection using Bayesian latent class analysis.
January 2023, Frontiers in veterinary science,
Anniken Jerre, and Anne Bang Nordstoga, and Katharine Rose Dean, and Ingrid Hunter Holmøy
Evaluation of the performance of three serological tests for diagnosis of Leishmania infantum infection in dogs using latent class analysis.
January 2020, Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria,
Copied contents to your clipboard!