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3H-spiroperidol, an antagonist of dopamine receptors in brain (striatum), was found to bind to human and rat platelet membrane preparations. The binding was rapid, reversible, saturable and specific. Unlabelled haloperidol displaced the specifically bound 3H-spiroperidol. Binding equilibrium was attained in 15 min at pH 7.4 and 37 degrees C. Scatchard analysis of 3H-spiroperidol binding revealed a single population of binding site with Kd of 7.6 nM in rat platelet membrane and Kd of 15 nM in human platelet membrane. Unlabelled 5-hydroxytryptamine produced no significant effect on 3H-spiroperidol binding to rat or human blood platelet membranes in the presence or absence of haloperidol. Some dopaminergic agents, known to inhibit spiroperidol binding in corpus striatum, also inhibited the same in rat and human blood platelet membranes under in vitro conditions. This study suggests the presence of specific 3H-spiroperidol binding sites in blood platelets.
A S Khanna, and
A K Agrawal, and
P K Seth
October 1987,
Biochemical pharmacology,
A S Khanna, and
A K Agrawal, and
P K Seth
October 1978,
European journal of pharmacology,
A S Khanna, and
A K Agrawal, and
P K Seth
October 1980,
Life sciences,
A S Khanna, and
A K Agrawal, and
P K Seth
January 1984,
Basic research in cardiology,
A S Khanna, and
A K Agrawal, and
P K Seth
May 1988,
Investigative ophthalmology & visual science,
A S Khanna, and
A K Agrawal, and
P K Seth
April 1980,
Life sciences,
A S Khanna, and
A K Agrawal, and
P K Seth
November 1986,
Life sciences,
A S Khanna, and
A K Agrawal, and
P K Seth
January 1989,
Life sciences,
A S Khanna, and
A K Agrawal, and
P K Seth
August 1978,
European journal of pharmacology,
A S Khanna, and
A K Agrawal, and
P K Seth
November 1980,
Life sciences,
