Biomaterial Database

Effect of pressure on the pre-steady-state kinetics of the hydrolysis of anilide substrates catalyzed by alpha-chymotrypsin. 1987

S Makimoto, and Y Taniguchi

The pre-steady-state of the hydrolysis of succinyl-L-alanyl-L-alanine p-nitroanilide (SA2pNA) and succinyl-L-alanine p-nitroanilide (SApNA) catalyzed by alpha-chymotrypsin in Tris buffer solution was measured up to 1.5 kbar at 25 degrees C. The pressure dependence of the formation of tetrahedral intermediate from the Michaelis complex on the rate constant (k1) gave activation volumes, delta V1 not equal to, of -33 +/- 2 cm3 X mol-1 for SA2pNA and -30 +/- 2 cm3 X mol-1 for SApNA. The reaction mechanism of alpha-chymotrypsin is discussed in terms of the activation volumes.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D011312	Pressure	A type of stress exerted uniformly in all directions. Its measure is the force exerted per unit area. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Pressures
D011485	Protein Binding	The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.	Plasma Protein Binding Capacity,Binding, Protein
D002918	Chymotrypsin	A serine endopeptidase secreted by the pancreas as its zymogen, CHYMOTRYPSINOGEN and carried in the pancreatic juice to the duodenum where it is activated by TRYPSIN. It selectively cleaves aromatic amino acids on the carboxyl side.	Alpha-Chymotrypsin Choay,Alphacutanée,Avazyme
D004151	Dipeptides	Peptides composed of two amino acid units.	Dipeptide
D006868	Hydrolysis	The process of cleaving a chemical compound by the addition of a molecule of water.
D000409	Alanine	A non-essential amino acid that occurs in high levels in its free state in plasma. It is produced from pyruvate by transamination. It is involved in sugar and acid metabolism, increases IMMUNITY, and provides energy for muscle tissue, BRAIN, and the CENTRAL NERVOUS SYSTEM.	Abufène,Alanine, L-Isomer,L-Alanine,Alanine, L Isomer,L Alanine,L-Isomer Alanine
D013379	Substrate Specificity	A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.	Specificities, Substrate,Specificity, Substrate,Substrate Specificities