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Newly developed techniques for the detection of sister-chromatid exchanges (SCE) require the substitution of 5-bromodeoxyuridine (BrdU) for thymidine in DNA. We investigated the possibility of interactions between BrdU and one mutagen--carcinogen, mitomycin C (MMC) for the induction of both chromosomal aberrations and SCE in human peripheral lymphocytes in culture. No effect on aberration yield was found. Neither comparisons between the yields of SCE by BrdU substitution and differential staining and those detected by tritiated thymidine incorporation and autoradiography nor between the yields of SCE for different levels of BrdU incorporation provided any evidence of synergism. It was found, however, that MMC persists in cultures and continues to increase SCE frequencies for about 30 h. It was also observed that some MMC-induced DNA lesions persist long enough so that some of those present prior to S phase of the first cell cycle cause additional SCE in the third cycle.
Y Ishii, and
M A Bender
January 1976,
Hereditas,
Y Ishii, and
M A Bender
August 1979,
Genetics,
Y Ishii, and
M A Bender
February 1980,
Cancer research,
Y Ishii, and
M A Bender
January 1984,
Basic life sciences,
Y Ishii, and
M A Bender
July 1974,
Experimental cell research,
Y Ishii, and
M A Bender
January 1980,
Chromosoma,
Y Ishii, and
M A Bender
May 1982,
Experientia,
Y Ishii, and
M A Bender
October 1982,
Indian journal of experimental biology,
Y Ishii, and
M A Bender
January 2003,
Teratogenesis, carcinogenesis, and mutagenesis,
Y Ishii, and
M A Bender
January 1993,
Acta biochimica Polonica,
