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Studies made on cultured skin fibroblasts obtained from a patient with primary cortisol resistance are described. Whole cell in vitro assays, using the patient's fibroblasts revealed a reduction in the dexamethasone binding capacity (7.86 +/- 0.73 fmol/micrograms DNA, mean +/- SD, n = 3; normal: 15.2 +/- 1.90 fmol/micrograms DNA, n = 8) and an apparently normal dissociation constant (3.69 +/- 0.15 nM; normal: 3.74 +/- 0.40 nM). In addition, the effects of glucocorticoids on DNA synthesis in these cells were examined. DNA synthesis was inhibited by dexamethasone both in normal fibroblasts and in the patient's cells, but the patient's cells were less sensitive to this inhibition, indicating resistance of the cells to glucocorticoid in vitro. These results suggest that the resistance of target tissues to glucocorticoids is due to the reduction in receptor number and that this is the primary defect in this new type of primary cortisol resistance in man.
M Gomi, and
S Iida, and
M Tsugawa, and
Y Itoh, and
K Moriwaki, and
H Fujii, and
S Yamashita, and
T Tamaki, and
S Tarui
November 1991,
The Journal of steroid biochemistry and molecular biology,
M Gomi, and
S Iida, and
M Tsugawa, and
Y Itoh, and
K Moriwaki, and
H Fujii, and
S Yamashita, and
T Tamaki, and
S Tarui
August 1987,
The Journal of clinical endocrinology and metabolism,
M Gomi, and
S Iida, and
M Tsugawa, and
Y Itoh, and
K Moriwaki, and
H Fujii, and
S Yamashita, and
T Tamaki, and
S Tarui
January 1990,
Transactions of the Association of American Physicians,
M Gomi, and
S Iida, and
M Tsugawa, and
Y Itoh, and
K Moriwaki, and
H Fujii, and
S Yamashita, and
T Tamaki, and
S Tarui
May 1993,
The Journal of clinical investigation,
M Gomi, and
S Iida, and
M Tsugawa, and
Y Itoh, and
K Moriwaki, and
H Fujii, and
S Yamashita, and
T Tamaki, and
S Tarui
June 1982,
The Journal of clinical investigation,
M Gomi, and
S Iida, and
M Tsugawa, and
Y Itoh, and
K Moriwaki, and
H Fujii, and
S Yamashita, and
T Tamaki, and
S Tarui
February 1989,
Journal of steroid biochemistry,
M Gomi, and
S Iida, and
M Tsugawa, and
Y Itoh, and
K Moriwaki, and
H Fujii, and
S Yamashita, and
T Tamaki, and
S Tarui
November 1986,
The Journal of clinical investigation,
M Gomi, and
S Iida, and
M Tsugawa, and
Y Itoh, and
K Moriwaki, and
H Fujii, and
S Yamashita, and
T Tamaki, and
S Tarui
June 1979,
Journal of steroid biochemistry,
M Gomi, and
S Iida, and
M Tsugawa, and
Y Itoh, and
K Moriwaki, and
H Fujii, and
S Yamashita, and
T Tamaki, and
S Tarui
March 1994,
Agents and actions,
M Gomi, and
S Iida, and
M Tsugawa, and
Y Itoh, and
K Moriwaki, and
H Fujii, and
S Yamashita, and
T Tamaki, and
S Tarui
January 1990,
Medicina cutanea ibero-latino-americana,
