BIOMAT Database Logo BIOMAT Database Logo

Enzymatic synthesis of RNA oligonucleotides. 1987

L Sharmeen, and J Taylor

Using SP6 RNA polymerase and various synthetic DNA oligomers as templates (12-65 b), we have obtained efficient synthesis of RNA transcripts without the need for either primer or promoter. Gel analysis of transcripts made from templates of known size demonstrated a predominant single species, together with some minor bands. For a given template the major band had the same 5'-nucleotide as predicted from the 3'-nucleotide of the template. This synthesis procedure makes it possible to efficiently and conveniently make labeled or unlabeled RNA from synthetic DNA oligonucleotides.

UI MeSH Term Description Entries
D009838 Oligodeoxyribonucleotides A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties. Oligodeoxynucleotide,Oligodeoxyribonucleotide,Oligodeoxynucleotides
D009843 Oligoribonucleotides A group of ribonucleotides (up to 12) in which the phosphate residues of each ribonucleotide act as bridges in forming diester linkages between the ribose moieties.
D012321 DNA-Directed RNA Polymerases Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992). DNA-Dependent RNA Polymerases,RNA Polymerases,Transcriptases,DNA-Directed RNA Polymerase,RNA Polymerase,Transcriptase,DNA Dependent RNA Polymerases,DNA Directed RNA Polymerase,DNA Directed RNA Polymerases,Polymerase, DNA-Directed RNA,Polymerase, RNA,Polymerases, DNA-Dependent RNA,Polymerases, DNA-Directed RNA,Polymerases, RNA,RNA Polymerase, DNA-Directed,RNA Polymerases, DNA-Dependent,RNA Polymerases, DNA-Directed
D013698 Templates, Genetic Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES. Genetic Template,Genetic Templates,Template, Genetic
D014158 Transcription, Genetic The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION. Genetic Transcription

Related Publications

L Sharmeen, and J Taylor
Initiator oligonucleotides for the combination of chemical and enzymatic RNA synthesis.
March 1992, Gene,
L Sharmeen, and J Taylor
Enzymatic synthesis of biphenyl-DNA oligonucleotides.
June 2020, Bioorganic & medicinal chemistry,
L Sharmeen, and J Taylor
Solid-phase enzymatic synthesis of oligonucleotides.
December 1999, Organic letters,
L Sharmeen, and J Taylor
[Enzymatic synthesis of oligonucleotides corresponding to the 3'-terminus of influenza virus RNA].
October 1983, Bioorganicheskaia khimiia,
L Sharmeen, and J Taylor
[Chemico-enzymatic method of synthesis of mixed oligonucleotides].
February 1987, Bioorganicheskaia khimiia,
L Sharmeen, and J Taylor
Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence.
August 1972, Nature: New biology,
L Sharmeen, and J Taylor
Enzymatic synthesis of RNA.
December 1960, Biochemical and biophysical research communications,
L Sharmeen, and J Taylor
Enzymatic Synthesis of Single-Stranded Clonal Pure Oligonucleotides.
January 2017, Methods in molecular biology (Clifton, N.J.),
L Sharmeen, and J Taylor
Enzymatic synthesis of phosphonomethyl oligonucleotides by therminator polymerase.
January 2007, Angewandte Chemie (International ed. in English),
L Sharmeen, and J Taylor
Enzymatic Synthesis of 7',5'-Bicyclo-DNA Oligonucleotides.
June 2017, Chemistry, an Asian journal,
Copied contents to your clipboard!