-transparent.png){kind=logo}
A capture enzyme immunoassay was developed for the detection of Mycoplasma bovis antigens in bull semen or preputial washings. IgG prepared from rabbits immunised with M bovis was passively adsorped to 96-well polystyrene plates. This antibody captured M bovis antigens which were then detected by using an IgG preparation from an immunised cow and murine monoclonal antibody to the bovine L-chain conjugated with horseradish peroxidase. The sensitivity of the assay was approximately 200 colour changing units (ccu)/ml and the specificity was excellent in that other species of mycoplasma, ureaplasma or acholeplasma did not react. A blind study of bull semen experimentally contaminated with M bovis detected all specimens with more than 200 ccu/ml.
K H Nielsen, and
R B Stewart, and
M M Garcia, and
M D Eaglesome
January 1983,
Acta veterinaria Scandinavica,
K H Nielsen, and
R B Stewart, and
M M Garcia, and
M D Eaglesome
April 1974,
The Cornell veterinarian,
K H Nielsen, and
R B Stewart, and
M M Garcia, and
M D Eaglesome
September 1994,
The Veterinary record,
K H Nielsen, and
R B Stewart, and
M M Garcia, and
M D Eaglesome
January 1988,
Revista Argentina de microbiologia,
K H Nielsen, and
R B Stewart, and
M M Garcia, and
M D Eaglesome
July 1986,
The Veterinary record,
K H Nielsen, and
R B Stewart, and
M M Garcia, and
M D Eaglesome
May 1985,
Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology,
K H Nielsen, and
R B Stewart, and
M M Garcia, and
M D Eaglesome
December 1994,
Veterinary microbiology,
K H Nielsen, and
R B Stewart, and
M M Garcia, and
M D Eaglesome
January 1983,
Acta veterinaria Scandinavica,
K H Nielsen, and
R B Stewart, and
M M Garcia, and
M D Eaglesome
January 1988,
Revue d'elevage et de medecine veterinaire des pays tropicaux,
K H Nielsen, and
R B Stewart, and
M M Garcia, and
M D Eaglesome
April 1982,
The Cornell veterinarian,
