In this work, the in vitro antioxidant and antimicrobial potential of hydroalcoholic extracts from the peel and seeds of jatobá fruit and the synergistic effect of their combination against pathogenic bacteria were evaluated. The 80% ethanol fruit seed extract showed a greater antioxidant potential and higher total phenolic content (5135.61 GAE/100 g dry residue) than the maximum value obtained from peel extract using 50% ethanol (2614.74 1 GAE/100 g dry residue). Moreover, 70% and 80% ethanol seed extracts inhibited most bacteria, especially Pseudomonas aeruginosa, with the lowest values of minimum inhibitory concentration (1.0 and 8.0 mg/mL), when compared with extracts from peel. Combining the 70% ethanol extracts from peels and seeds reduced their inhibitory concentration by about 4 to 32 times against Bacillus cereus, Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, P. aeruginosa and Salmonella enteritidis, when compared to each extract alone. Extracts from the peel and seeds of the jatobá fruit showed potential as natural antioxidants and antimicrobials.