Hyperbolic material enhanced scattering nanoscopy for label-free super-resolution imaging. 2022

Yeon Ui Lee, and Shilong Li, and G Bimananda M Wisna, and Junxiang Zhao, and Yuan Zeng, and Andrea R Tao, and Zhaowei Liu
Department of Electrical and Computer Engineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.

Fluorescence super-resolution microscopy has, over the last two decades, been extensively developed to access deep-subwavelength nanoscales optically. Label-free super-resolution technologies however have only achieved a slight improvement compared to the diffraction limit. In this context, we demonstrate a label-free imaging method, i.e., hyperbolic material enhanced scattering (HMES) nanoscopy, which breaks the diffraction limit by tailoring the light-matter interaction between the specimens and a hyperbolic material substrate. By exciting the highly confined evanescent hyperbolic polariton modes with dark-field detection, HMES nanoscopy successfully shows a high-contrast scattering image with a spatial resolution around 80 nm. Considering the wavelength at 532 nm and detection optics with a 0.6 numerical aperture (NA) objective lens, this value represents a 5.5-fold resolution improvement beyond the diffraction limit. HMES provides capabilities for super-resolution imaging where fluorescence is not available or challenging to apply.

UI MeSH Term Description Entries
D008856 Microscopy, Fluorescence Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence

Related Publications

Yeon Ui Lee, and Shilong Li, and G Bimananda M Wisna, and Junxiang Zhao, and Yuan Zeng, and Andrea R Tao, and Zhaowei Liu
June 2022, Annual review of analytical chemistry (Palo Alto, Calif.),
Yeon Ui Lee, and Shilong Li, and G Bimananda M Wisna, and Junxiang Zhao, and Yuan Zeng, and Andrea R Tao, and Zhaowei Liu
June 2021, Nature communications,
Yeon Ui Lee, and Shilong Li, and G Bimananda M Wisna, and Junxiang Zhao, and Yuan Zeng, and Andrea R Tao, and Zhaowei Liu
July 2016, Scientific reports,
Yeon Ui Lee, and Shilong Li, and G Bimananda M Wisna, and Junxiang Zhao, and Yuan Zeng, and Andrea R Tao, and Zhaowei Liu
August 2016, Microscopy research and technique,
Yeon Ui Lee, and Shilong Li, and G Bimananda M Wisna, and Junxiang Zhao, and Yuan Zeng, and Andrea R Tao, and Zhaowei Liu
January 2013, Scientific reports,
Yeon Ui Lee, and Shilong Li, and G Bimananda M Wisna, and Junxiang Zhao, and Yuan Zeng, and Andrea R Tao, and Zhaowei Liu
November 2018, Journal of biomedical optics,
Yeon Ui Lee, and Shilong Li, and G Bimananda M Wisna, and Junxiang Zhao, and Yuan Zeng, and Andrea R Tao, and Zhaowei Liu
September 2015, Scientific reports,
Yeon Ui Lee, and Shilong Li, and G Bimananda M Wisna, and Junxiang Zhao, and Yuan Zeng, and Andrea R Tao, and Zhaowei Liu
January 2015, Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology,
Yeon Ui Lee, and Shilong Li, and G Bimananda M Wisna, and Junxiang Zhao, and Yuan Zeng, and Andrea R Tao, and Zhaowei Liu
January 2023, Methods in molecular biology (Clifton, N.J.),
Yeon Ui Lee, and Shilong Li, and G Bimananda M Wisna, and Junxiang Zhao, and Yuan Zeng, and Andrea R Tao, and Zhaowei Liu
February 2018, Scientific reports,
Copied contents to your clipboard!