Biomaterial Database

Determination of Total Sennosides and Sennosides A, B, and A1 in Senna Leaflets, Pods, and Tablets by Two-Dimensional qNMR. 2022

Serhat Sezai Çiçek, and Calisto Moreno Cardenas, and Ulrich Girreser

Pharmazeutisches Institut, Abteilung Pharmazeutische Biologie, Christian-Albrechts-Universität zu Kiel, Gutenbergstraße 76, 24118 Kiel, Germany.

In the present work, a two-dimensional qNMR method for the determination of sennosides was established. Using band-selective HSQC and the cross correlations of the characteristic 10-10' bonds, we quantified the total amount of the value-determining dianthranoids in five minutes, thus, rendering the method not only fast, but also specific and stability indicating. The validation of the method revealed excellent accuracy (recovery rates of 98.5 to 103%), precision (RSD values of 3.1%), and repeatability (2.2%) and demonstrated the potential of 2D qNMR in the quality control of medicinal plants. In a second method, the use of 2D qNMR for the single analysis of sennosides A, B, and A1 was evaluated with acceptable measurement times (31 min), accuracy (93.8%), and repeatability (5.4% and 5.6%) for the two major purgatives sennoside A and B. However, the precision for sennoside B and A1 was not satisfactory, mainly due to the low resolution of the HSQC signals of the two compounds.

UI	MeSH Term	Description	Entries
D002400	Cathartics	Agents that are used to stimulate evacuation of the bowels.	Bowel Evacuant,Bowel Preparation Solution,Cathartic,Purgative,Purgatives,Bowel Evacuants,Bowel Preparation Solutions,Evacuant, Bowel,Evacuants, Bowel,Preparation Solution, Bowel,Preparation Solutions, Bowel,Solution, Bowel Preparation,Solutions, Bowel Preparation
D000081226	Sennosides	Medications derived from SENNA EXTRACT that are used to treat CONSTIPATION.	Senna Glycoside,Sennoside,Sennoside A Calcium,Sennoside A Calcium and Sennoside B Calcium,Sennoside A&B,Sennoside A&B, Calcium Salt,Sennoside A+B Calcium,Sennoside A, Calcium Salt,Sennoside A, Calcium Salt (1:1),Sennoside B Calcium,Sennoside B, Calcium Salt,Sennosides A and B,Sennosides A and B Acids,Pursennid,Senokot
D000880	Anthraquinones	Compounds based on ANTHRACENES which contain two KETONES in any position. Substitutions can be in any position except on the ketone groups.	Anthracenedione,Anthracenediones,Anthranoid,Anthraquinone,Anthraquinone Compound,Anthraquinone Derivative,Dianthraquinones,Dianthrones,Anthranoids,Anthraquinone Compounds,Anthraquinone Derivatives,Compound, Anthraquinone,Derivative, Anthraquinone
D012676	Senna Extract	Preparations of SENNA PLANT. They contain sennosides, which are anthraquinone type CATHARTICS and are used in many different preparations as laxatives.	Senna Leaf,Senna Pods,Sennae Folium,Sennae Fructus,Extract, Senna,Folium, Sennae,Fructus, Sennae,Leaf, Senna,Pods, Senna
D013607	Tablets	Solid dosage forms, of varying weight, size, and shape, which may be molded or compressed, and which contain a medicinal substance in pure or diluted form. (Dorland, 28th ed)	Tablet
D029846	Senna Plant	A plant genus of the family FABACEAE. SENNA EXTRACT is obtained from members of this genus. Members contain ANTHRAQUINONES and have been an ingredient in laxatives (CATHARTICS). Many species of the CASSIA genus have been reclassified into this genus. This bush should not be confused with the Cassia tree (CINNAMOMUM).	Cassia acutifolia,Coffee Senna,Indian Senna,Cassia angustifolia,Cassia occidentalis,Cassia senna,Senna alexandrina,Senna occidentalis,Coffee Sennas,Indian Sennas,Plant, Senna,Senna Plants,Senna, Coffee,Senna, Indian