Ecological security is crucial for regional sustainable development; however, as modern urbanization highlights ecological security challenges, major challenges have arisen. In this paper, we take the ecological region around Taihu Lake, China, as a typical research site, extract important ecological sources and key nodes using morphological spatial pattern analysis (MSPA) and circuit theory, and propose a regulatory framework for the ecological security pattern (ESP) of the ecological region based on the spatial characteristics of sources, corridors, and nodes. We obtained the following results: (1) The ESP includes 20 ecological sources, 37 ecological corridors, 36 critical ecological protection nodes, and 24 key ecological restoration nodes. (2) Most ecological sources are large and concentrated in western Zhejiang and west of Taihu Lake, which are both important ecological sources and ecological resistance surfaces. (3) The ecological corridors spread east, west, and south from Taihu Lake, with high network connectivity. (4) Shanghai serves as the central node, with the Su-Xi-Chang town cluster and the Qiantang River town cluster serving as the extension axes for the ecological resistance hot-spot area. The center of the elliptical ecological resistance surface (standard deviation) lies in Suzhou City, located on the east shore of Taihu Lake. (5) Ecological nodes were mostly located in ecological corridors or junctions. A "four zones and one belt" pattern is suggested in order to make the land around Taihu Lake more connected and stable ecologically. This study can be used as a guide for building and improving an ecological safety network.