-transparent.png){kind=logo}
Despite a decrease in the extent to which confluent monolayers of late compared to early passage CF3 human diploid fibroblasts can be stimulated to proliferate, the time course of DNA synthesis onset is similar regardless of the in vitro age of the cells. A parallel and stoichiometric relationship is maintained between the rate of DNA synthesis and the cellular levels of histone mRNA independent of the age of the cell cultures. Furthermore, DNA synthesis and cellular histone mRNA levels decline in a coordinate manner after inhibition of DNA replication by hydroxyurea treatment. These results indicate that while the proliferative activity of human diploid fibroblasts decreases with passage in culture, those cells that retain the ability to proliferate continue to exhibit a tight coupling of DNA replication and histone gene expression.
G Zambetti, and
R Dell'Orco, and
G Stein, and
J Stein
November 1986,
Mechanisms of ageing and development,
G Zambetti, and
R Dell'Orco, and
G Stein, and
J Stein
November 2017,
Oncotarget,
G Zambetti, and
R Dell'Orco, and
G Stein, and
J Stein
March 1990,
Journal of gerontology,
G Zambetti, and
R Dell'Orco, and
G Stein, and
J Stein
November 2022,
Molecular and cellular biology,
G Zambetti, and
R Dell'Orco, and
G Stein, and
J Stein
September 2012,
Osteoarthritis and cartilage,
G Zambetti, and
R Dell'Orco, and
G Stein, and
J Stein
November 2017,
Experimental cell research,
G Zambetti, and
R Dell'Orco, and
G Stein, and
J Stein
January 2013,
PloS one,
G Zambetti, and
R Dell'Orco, and
G Stein, and
J Stein
June 2004,
Cell cycle (Georgetown, Tex.),
G Zambetti, and
R Dell'Orco, and
G Stein, and
J Stein
January 1989,
Journal of cellular biochemistry,
G Zambetti, and
R Dell'Orco, and
G Stein, and
J Stein
July 1991,
Experimental cell research,
