Simultaneous determination of nalidixic acid, oxolinic acid and piromidic acid in fish by high-performance liquid chromatography with fluorescence and UV detection. 1987

M Horie, and K Saito, and Y Hoshino, and N Nose, and E Mochizuki, and H Nakazawa
Saitama Prefectural Institute of Public Health, Japan.

A simple and rapid method for the simultaneous determination of nalidixic acid (NA), oxolinic acid (OXA) and piromidic acid (PMA) in cultured fish has been developed by high-performance liquid chromatography (HPLC). The drugs were extracted with 0.1% metaphosphoric acid-methanol (6:4), followed by a Sep-Pak C18 clean-up procedure. The HPLC separation was carried out on a Kaseisorb LC ODS 300-5 column (25 cm x 4.6 mm I.D.) using 5 mM phosphate buffer-acetonitrile (6:4) as a mobile phase. A fluorescence detector was used for NA and OXA at the excitation wavelength of 325 nm and the emission wavelength of 365 nm and an ultraviolet detector at 280 nm for PMA. The calibration graphs were rectilinear from 1 to 10 ng for OXA, from 2 to 20 ng for NA and PMA. The recoveries of NA, OXA and PMA added to fish were 81.5-85.3, 83.7-88.7 and 80.9-84.9%, respectively, with high accuracy. The limits of detection were 0.01 micrograms/g for each drug.

UI MeSH Term Description Entries
D007202 Indicators and Reagents Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499) Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D009268 Nalidixic Acid A synthetic 1,8-naphthyridine antimicrobial agent with a limited bacteriocidal spectrum. It is an inhibitor of the A subunit of bacterial DNA GYRASE. Nalidixin,Nalidixate Sodium,Nalidixate Sodium Anhydrous,Nevigramon,Sodium Nalidixic Acid, Anhydrous,Sodium Nalidixic Acid, Monohydrate,Acid, Nalidixic,Anhydrous, Nalidixate Sodium,Sodium Anhydrous, Nalidixate,Sodium, Nalidixate
D009539 Nicotinic Acids 2-, 3-, or 4-Pyridinecarboxylic acids. Pyridine derivatives substituted with a carboxy group at the 2-, 3-, or 4-position. The 3-carboxy derivative (NIACIN) is active as a vitamin. Acids, Nicotinic
D010093 Oxolinic Acid Synthetic antimicrobial related to NALIDIXIC ACID and used in URINARY TRACT INFECTIONS. Gramurin,Sodium Oxolinate,Acid, Oxolinic,Oxolinate, Sodium
D010893 Piromidic Acid Antibacterial against mainly gram negative organisms. It is used for urinary tract and intestinal infections. Acid, Piromidic
D002347 Carps Common name for a number of different species of fish in the family Cyprinidae. This includes, among others, the common carp, crucian carp, grass carp, and silver carp. Carassius carassius,Crucian Carp,Cyprinus,Grass Carp,Carp,Ctenopharyngodon idellus,Cyprinus carpio,Hypophthalmichthys molitrix,Koi Carp,Silver Carp,Carp, Crucian,Carp, Grass,Carp, Koi,Carp, Silver,Carps, Crucian,Carps, Grass,Carps, Silver,Crucian Carps,Grass Carps,Silver Carps
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D005399 Fishes A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013050 Spectrometry, Fluorescence Measurement of the intensity and quality of fluorescence. Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence

Related Publications

M Horie, and K Saito, and Y Hoshino, and N Nose, and E Mochizuki, and H Nakazawa
February 1987, Journal of chromatography,
M Horie, and K Saito, and Y Hoshino, and N Nose, and E Mochizuki, and H Nakazawa
August 1990, Journal of chromatography,
M Horie, and K Saito, and Y Hoshino, and N Nose, and E Mochizuki, and H Nakazawa
January 1998, Journal of AOAC International,
M Horie, and K Saito, and Y Hoshino, and N Nose, and E Mochizuki, and H Nakazawa
February 1997, Journal of chromatography. B, Biomedical sciences and applications,
M Horie, and K Saito, and Y Hoshino, and N Nose, and E Mochizuki, and H Nakazawa
December 1989, Journal of chromatography,
M Horie, and K Saito, and Y Hoshino, and N Nose, and E Mochizuki, and H Nakazawa
March 2009, Se pu = Chinese journal of chromatography,
M Horie, and K Saito, and Y Hoshino, and N Nose, and E Mochizuki, and H Nakazawa
November 2000, Journal of chromatography. B, Biomedical sciences and applications,
M Horie, and K Saito, and Y Hoshino, and N Nose, and E Mochizuki, and H Nakazawa
December 2008, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
M Horie, and K Saito, and Y Hoshino, and N Nose, and E Mochizuki, and H Nakazawa
July 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
M Horie, and K Saito, and Y Hoshino, and N Nose, and E Mochizuki, and H Nakazawa
January 1991, Journal - Association of Official Analytical Chemists,
Copied contents to your clipboard!