BACKGROUND The anterior and overall Bolton ratios and their application in orthodontics are widely known. However, little has been reported about the posterior Bolton ratio, how it is affected by the extraction of posterior teeth, and its application in orthodontic treatment planning. This study aimed to investigate how extracting maxillary first and mandibular second premolars affects the posterior Bolton ratio. METHODS The sample included 55 patients with Class I occlusion within 1 standard deviation of ideal anterior and overall Bolton ratios. The digitized models were subjected to virtual extraction of maxillary first premolars and mandibular second premolars and setup of posterior teeth in ideal occlusion. If space closure compromised occlusion, the teeth were moved to achieve ideal cusp-fossa or cusp-marginal ridge occlusion. The ideal setups were measured for residual interproximal spacing. Statistical analysis used R statistical software (version 2018; R Core Team, Vienna, Austria). RESULTS The ideal nonextraction posterior Bolton ratio was determined from the sample to be 105.77 ± 1.99%. The ideal expected posterior Bolton ratio for maxillary first and mandibular second premolar extraction patients was 106.52 ± 2.52%. This significantly differed from the expected posterior Bolton ratio for the 4 first premolar extractions. Patients finished with an average of 1.28 mm net residual spacing between mandibular first premolars and first molars; 38.2% of patients finished with at least 1.5 mm of residual space, and 9.1% of patients finished with at least 2 mm of residual space. CONCLUSIONS A patient with ideal anterior, posterior, and overall Bolton ratios treated with maxillary first and mandibular second premolar extractions to ideal occlusion will likely finish with some spacing in the mandibular dentition.