In-depth interpretation of aptamer-based sensing on electrode: Dual-mode electrochemical-photoelectrochemical sensor for the ratiometric detection of patulin. 2023

Shuda Liu, and Shuyun Meng, and Meng Wang, and Wenjia Li, and Na Dong, and Dong Liu, and Yuye Li, and Tianyan You
Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.

Electrochemical aptasensors have been extensively used to quantify food contaminants (e.g., mycotoxin) by using high-affinity aptamer for target recognition. Yet, analytical performance of aptasensors using different aptamers can be varied for the same target. Here, four aptamers with different sequences (i.e., A22, A34, A42, and A45) of patulin (PAT) were selected to estimate sensing behaviors at electrodes with electrochemical (EC) and photoelectrochemical (PEC) assays. Synergistic effect of steric hindrance and electron transfer distance was found to significantly affect EC and PEC response for PAT at aptasensors fabricated with A22, A34, A42, or A45. Eventually, A22 emerged to be the optimal aptamer for aptasensing, despite the highest affinity of A42 to PAT. The A22-based EC-PEC dual-mode ratiometric aptasensor offered a linear range of 50 fg mL-1 - 500 ng mL-1 with a detection limit of 30 fg mL-1 for PAT, and it was applied to apple product (i.e., juice, puree) analysis.

UI MeSH Term Description Entries
D010365 Patulin 4-Hydroxy-4H-furo(3,2-c)pyran-2(6H)-one. A mycotoxin produced by several species of Aspergillus and Penicillium. It is found in unfermented apple and grape juice and field crops. It has antibiotic properties and has been shown to be carcinogenic and mutagenic and causes chromosome damage in biological systems.
D004566 Electrodes Electric conductors through which electric currents enter or leave a medium, whether it be an electrolytic solution, solid, molten mass, gas, or vacuum. Anode,Anode Materials,Cathode,Cathode Materials,Anode Material,Anodes,Cathode Material,Cathodes,Electrode,Material, Anode,Material, Cathode
D006046 Gold A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
D015374 Biosensing Techniques Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal. Bioprobes,Biosensors,Electrodes, Enzyme,Biosensing Technics,Bioprobe,Biosensing Technic,Biosensing Technique,Biosensor,Electrode, Enzyme,Enzyme Electrode,Enzyme Electrodes,Technic, Biosensing,Technics, Biosensing,Technique, Biosensing,Techniques, Biosensing
D052157 Aptamers, Nucleotide Nucleotide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity. DNA Aptamer,DNA Aptamers,RNA Aptamers,Rna Aptamer,Nucleotide Aptamers,Oligonucleotide Ligands, DNA,Oligonucleotide Ligands, RNA,Aptamer, DNA,Aptamer, Rna,Aptamers, DNA,Aptamers, RNA,DNA Oligonucleotide Ligands,RNA Oligonucleotide Ligands
D055664 Electrochemical Techniques The utilization of an electrical current to measure, analyze, or alter chemicals or chemical reactions in solution, cells, or tissues. Electrochemical Technics,Electrochemical Technic,Electrochemical Technique,Technic, Electrochemical,Technics, Electrochemical,Technique, Electrochemical,Techniques, Electrochemical
D057230 Limit of Detection Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure. Limits of Detection,Detection Limit,Detection Limits
D027845 Malus A plant genus in the family ROSACEAE, order Rosales, subclass Rosidae. It is best known as a source of the edible fruit (apple) and is cultivated in temperate climates worldwide. Apple,Apple Tree,Crab Apple,Malus domestica,Apple Trees,Apple, Crab,Apples,Apples, Crab,Crab Apples,Tree, Apple,Trees, Apple

Related Publications

Shuda Liu, and Shuyun Meng, and Meng Wang, and Wenjia Li, and Na Dong, and Dong Liu, and Yuye Li, and Tianyan You
February 2024, Toxicon : official journal of the International Society on Toxinology,
Shuda Liu, and Shuyun Meng, and Meng Wang, and Wenjia Li, and Na Dong, and Dong Liu, and Yuye Li, and Tianyan You
October 2022, Mikrochimica acta,
Shuda Liu, and Shuyun Meng, and Meng Wang, and Wenjia Li, and Na Dong, and Dong Liu, and Yuye Li, and Tianyan You
December 2023, Analytical chemistry,
Shuda Liu, and Shuyun Meng, and Meng Wang, and Wenjia Li, and Na Dong, and Dong Liu, and Yuye Li, and Tianyan You
March 2022, Analytica chimica acta,
Shuda Liu, and Shuyun Meng, and Meng Wang, and Wenjia Li, and Na Dong, and Dong Liu, and Yuye Li, and Tianyan You
November 2020, The Analyst,
Shuda Liu, and Shuyun Meng, and Meng Wang, and Wenjia Li, and Na Dong, and Dong Liu, and Yuye Li, and Tianyan You
June 2024, Biosensors & bioelectronics,
Shuda Liu, and Shuyun Meng, and Meng Wang, and Wenjia Li, and Na Dong, and Dong Liu, and Yuye Li, and Tianyan You
January 2021, Journal of colloid and interface science,
Shuda Liu, and Shuyun Meng, and Meng Wang, and Wenjia Li, and Na Dong, and Dong Liu, and Yuye Li, and Tianyan You
May 2024, Toxicon : official journal of the International Society on Toxinology,
Shuda Liu, and Shuyun Meng, and Meng Wang, and Wenjia Li, and Na Dong, and Dong Liu, and Yuye Li, and Tianyan You
November 2021, Langmuir : the ACS journal of surfaces and colloids,
Shuda Liu, and Shuyun Meng, and Meng Wang, and Wenjia Li, and Na Dong, and Dong Liu, and Yuye Li, and Tianyan You
March 2023, Food chemistry,
Copied contents to your clipboard!