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A 50-base synthetic oligodeoxynucleotide complementary to a portion of mouse nerve growth factor (NGF) mRNA was used as a probe for analysis of the expression of NGF gene. Northern blot analysis showed the presence of a major 1.3 kb transcript, which was identical in size to mouse NGF mRNA, in both C6Bu1 cells and rat astrocytes cultured from newborn rat brain. Further, the rearrangement of DNA sequence in and around the NGF gene locus of C6Bu1 cells was not detected by Southern blot analysis. These results indicate the expression of NGF mRNA in both C6Bu1 cells and astrocytes from rat brain, suggesting that astrocytes may produce NGF protein in the rat brain, especially in developing rat brain.
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F Hishinuma, and
R Kuwano, and
Y Takahashi, and
T Amano
June 1974,
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T Yamakuni, and
F Ozawa, and
F Hishinuma, and
R Kuwano, and
Y Takahashi, and
T Amano
January 1992,
Experimental brain research,
T Yamakuni, and
F Ozawa, and
F Hishinuma, and
R Kuwano, and
Y Takahashi, and
T Amano
December 1993,
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T Yamakuni, and
F Ozawa, and
F Hishinuma, and
R Kuwano, and
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T Amano
August 1978,
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T Yamakuni, and
F Ozawa, and
F Hishinuma, and
R Kuwano, and
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T Amano
August 2005,
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T Yamakuni, and
F Ozawa, and
F Hishinuma, and
R Kuwano, and
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T Amano
September 1997,
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T Yamakuni, and
F Ozawa, and
F Hishinuma, and
R Kuwano, and
Y Takahashi, and
T Amano
February 1990,
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T Yamakuni, and
F Ozawa, and
F Hishinuma, and
R Kuwano, and
Y Takahashi, and
T Amano
December 1988,
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T Yamakuni, and
F Ozawa, and
F Hishinuma, and
R Kuwano, and
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T Amano
January 1994,
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T Yamakuni, and
F Ozawa, and
F Hishinuma, and
R Kuwano, and
Y Takahashi, and
T Amano
October 1988,
Brain research,
