BIOMAT Database Logo BIOMAT Database Logo

A species-specific DNA probe for the detection of Mycoplasma gallisepticum. 1987

M Santha, and K Burg, and I Rasko, and L Stipkovits
Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest.

An 800-base-pair DNA fragment from a partial genomic library of Mycoplasma gallisepticum was selected and used as a probe for the selective detection of this avian pathogen. The specificity and sensitivity of this probe were demonstrated by using dot blot and Southern hybridizations.

UI MeSH Term Description Entries
D009174 Mycoplasma A genus of gram-negative, mostly facultatively anaerobic bacteria in the family MYCOPLASMATACEAE. The cells are bounded by a PLASMA MEMBRANE and lack a true CELL WALL. Its organisms are pathogens found on the MUCOUS MEMBRANES of humans, ANIMALS, and BIRDS. Eperythrozoon,Haemobartonella,Mycoplasma putrefaciens,PPLO,Pleuropneumonia-Like Organisms,Pleuropneumonia Like Organisms
D009175 Mycoplasma Infections Infections with species of the genus MYCOPLASMA. Eperythrozoonosis,Infections, Mycoplasma,Eperythrozoonoses,Infection, Mycoplasma,Mycoplasma Infection
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D011201 Poultry Diseases Diseases of birds which are raised as a source of meat or eggs for human consumption and are usually found in barnyards, hatcheries, etc. The concept is differentiated from BIRD DISEASES which is for diseases of birds not considered poultry and usually found in zoos, parks, and the wild. Disease, Poultry,Diseases, Poultry,Poultry Disease
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species

Related Publications

M Santha, and K Burg, and I Rasko, and L Stipkovits
Species-specific biotinylated DNA probe for the detection of Mycoplasma gallisepticum.
September 1988, Molecular and cellular probes,
M Santha, and K Burg, and I Rasko, and L Stipkovits
Detection of Mycoplasma gallisepticum infection in field samples using a species-specific DNA probe.
January 1993, Avian diseases,
M Santha, and K Burg, and I Rasko, and L Stipkovits
A Mycoplasma gallisepticum strain-specific DNA probe.
January 1987, Avian diseases,
M Santha, and K Burg, and I Rasko, and L Stipkovits
The use of a specific dna probe for detection of Mycoplasma gallisepticum in field outbreaks.
July 1989, Avian pathology : journal of the W.V.P.A,
M Santha, and K Burg, and I Rasko, and L Stipkovits
A species-specific DNA probe for the detection of Mycoplasma agalactiae.
August 1994, Veterinary microbiology,
M Santha, and K Burg, and I Rasko, and L Stipkovits
Mycoplasma iowae species-specific DNA probe.
October 1990, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
M Santha, and K Burg, and I Rasko, and L Stipkovits
Mycoplasma gallisepticum species and strain-specific recombinant DNA probes.
January 1989, Avian pathology : journal of the W.V.P.A,
M Santha, and K Burg, and I Rasko, and L Stipkovits
A DNA probe for specific detection of Mycoplasma pulmonis.
July 1989, Jikken dobutsu. Experimental animals,
M Santha, and K Burg, and I Rasko, and L Stipkovits
Development of a species-specific DNA probe for Mycoplasma capricolum.
September 1992, Veterinary microbiology,
M Santha, and K Burg, and I Rasko, and L Stipkovits
Development of a biotinylated probe for the rapid detection of Mycoplasma gallisepticum.
June 1987, Israel journal of medical sciences,
Copied contents to your clipboard!