Biomaterial Database

Cross-reactions between Mycoplasma hyopneumoniae and Mycoplasma flocculare--practical implications for the serodiagnosis of mycoplasmal pneumonia of swine. 1987

C H Armstrong, and M J Freeman, and L Sands-Freeman

Department of Veterinary Microbiology, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907.

Previous studies using hyperimmune antisera revealed significant serologic cross-reactions between Mycoplasma hyopneumoniae and M. flocculare. These findings, coupled with observations that M. flocculare infection may be widespread, indicated that antibodies to M. flocculare might be common in swine sera and thus cause nonspecific reactions in serodiagnostic tests for mycoplasmal pneumonia of swine (MPS). Information reported here indicates that this premise may be invalid. Twenty-one swine were infected experimentally with M. flocculare. Sera were collected every 2 weeks and tested against M. flocculare and M. hyopneumoniae antigens in an enzyme-linked immunosorbent assay (ELISA). Immunoassays against M. flocculare revealed that swine were slow to seroconvert, and that seroconversion was characterized by low titers. Significantly, none of the sera reacted with M. hyopneumoniae. M. hyopneumoniae reference antisera, and sera from swine naturally infected with M. hyopneumoniae and M. flocculare were also evaluated by an antibody inhibition procedure, i.e., sera were mixed with M. hyopneumoniae or M. flocculare antigen, incubated overnight and tested against M. hyopneumoniae antigen in the ELISA. Treatment with M. hyopneumoniae antigen markedly reduced or abolished seroreactivity, whereas treatment with M. flocculare antigen had little effect. These findings suggest that swine infected with M. flocculare usually do not develop sufficient levels of antibodies to detract from the specificity of the ELISA used to diagnose MPS.

UI	MeSH Term	Description	Entries
D009174	Mycoplasma	A genus of gram-negative, mostly facultatively anaerobic bacteria in the family MYCOPLASMATACEAE. The cells are bounded by a PLASMA MEMBRANE and lack a true CELL WALL. Its organisms are pathogens found on the MUCOUS MEMBRANES of humans, ANIMALS, and BIRDS.	Eperythrozoon,Haemobartonella,Mycoplasma putrefaciens,PPLO,Pleuropneumonia-Like Organisms,Pleuropneumonia Like Organisms
D009175	Mycoplasma Infections	Infections with species of the genus MYCOPLASMA.	Eperythrozoonosis,Infections, Mycoplasma,Eperythrozoonoses,Infection, Mycoplasma,Mycoplasma Infection
D011014	Pneumonia	Infection of the lung often accompanied by inflammation.	Experimental Lung Inflammation,Lobar Pneumonia,Lung Inflammation,Pneumonia, Lobar,Pneumonitis,Pulmonary Inflammation,Experimental Lung Inflammations,Inflammation, Experimental Lung,Inflammation, Lung,Inflammation, Pulmonary,Inflammations, Lung,Inflammations, Pulmonary,Lobar Pneumonias,Lung Inflammation, Experimental,Lung Inflammations,Lung Inflammations, Experimental,Pneumonias,Pneumonias, Lobar,Pneumonitides,Pulmonary Inflammations
D003429	Cross Reactions	Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.	Cross Reaction,Reaction, Cross,Reactions, Cross
D005189	False Positive Reactions	Positive test results in subjects who do not possess the attribute for which the test is conducted. The labeling of healthy persons as diseased when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)	False Positive Reaction,Positive Reaction, False,Positive Reactions, False,Reaction, False Positive,Reactions, False Positive
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000907	Antibodies, Bacterial	Immunoglobulins produced in a response to BACTERIAL ANTIGENS.	Bacterial Antibodies
D012698	Serologic Tests	Diagnostic procedures involving immunoglobulin reactions.	Serodiagnosis,Serologic Test,Serological Tests,Test, Serologic,Tests, Serologic,Serodiagnoses,Serological Test,Test, Serological,Tests, Serological
D013045	Species Specificity	The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.	Species Specificities,Specificities, Species,Specificity, Species
D013552	Swine	Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).	Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog