BIOMAT Database Logo BIOMAT Database Logo

Biochemical characterization of plant aromatic aminotransferases. 2023

Kaan Koper, and Shogo Hataya, and Andrew G Hall, and Taichi E Takasuka, and Hiroshi A Maeda
Department of Botany, University of Wisconsin-Madison, Madison, WI, United States. Electronic address: koper@wisc.edu.

Aromatic aminotransferases (Aro ATs) are pyridoxal-5-phosphate (PLP)-dependent enzymes that catalyze the transamination reactions of an aromatic amino acid (AAA) or a keto acid. Aro ATs are involved in biosynthesis or degradation of AAAs and play important functions in controlling the production of plant hormones and secondary metabolites, such as auxin, tocopherols, flavonoids, and lignin. Most Aro ATs show substrate promiscuity and can accept multiple aromatic and non-aromatic amino and keto acid substrates, which complicates and limits our understanding of their in planta functions. Considering the critical roles Aro ATs play in plant primary and secondary metabolism, it is important to accurately determine substrate specificity and kinetic properties of Aro ATs. This chapter describes various methodologies of protein expression, purification and enzymatic assays, which can be used for biochemical characterization of Aro ATs.

UI MeSH Term Description Entries
D007651 Keto Acids Carboxylic acids that contain a KETONE group. Oxo Acids,Oxoacids,Acids, Keto,Acids, Oxo
D011732 Pyridoxal Phosphate This is the active form of VITAMIN B 6 serving as a coenzyme for synthesis of amino acids, neurotransmitters (serotonin, norepinephrine), sphingolipids, aminolevulinic acid. During transamination of amino acids, pyridoxal phosphate is transiently converted into pyridoxamine phosphate (PYRIDOXAMINE). Pyridoxal 5-Phosphate,Pyridoxal-P,Phosphate, Pyridoxal,Pyridoxal 5 Phosphate,Pyridoxal P
D000637 Transaminases A subclass of enzymes of the transferase class that catalyze the transfer of an amino group from a donor (generally an amino acid) to an acceptor (generally a 2-keto acid). Most of these enzymes are pyridoxyl phosphate proteins. (Dorland, 28th ed) EC 2.6.1. Aminotransferase,Aminotransferases,Transaminase
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D024322 Amino Acids, Aromatic Amino acids containing an aromatic side chain. Aromatic Amino Acids,Amino Acid, Aromatic,Aromatic Amino Acid

Related Publications

Kaan Koper, and Shogo Hataya, and Andrew G Hall, and Taichi E Takasuka, and Hiroshi A Maeda
Characterization of aromatic aminotransferases from Ephedra sinica Stapf.
May 2016, Amino acids,
Kaan Koper, and Shogo Hataya, and Andrew G Hall, and Taichi E Takasuka, and Hiroshi A Maeda
Characterization of aromatic aminotransferases from the hyperthermophilic archaeon Thermococcus litoralis.
March 1994, European journal of biochemistry,
Kaan Koper, and Shogo Hataya, and Andrew G Hall, and Taichi E Takasuka, and Hiroshi A Maeda
Tryptophan degradation in Saccharomyces cerevisiae: characterization of two aromatic aminotransferases.
December 1982, Archives of microbiology,
Kaan Koper, and Shogo Hataya, and Andrew G Hall, and Taichi E Takasuka, and Hiroshi A Maeda
Aromatic aminotransferases in coryneform bacteria.
November 1979, Journal of bacteriology,
Kaan Koper, and Shogo Hataya, and Andrew G Hall, and Taichi E Takasuka, and Hiroshi A Maeda
A multispecific quintet of aromatic aminotransferases that overlap different biochemical pathways in Pseudomonas aeruginosa.
November 1982, The Journal of biological chemistry,
Kaan Koper, and Shogo Hataya, and Andrew G Hall, and Taichi E Takasuka, and Hiroshi A Maeda
Differences in properties between aromatic amino acid: aromatic keto acid aminotransferases and aromatic amino acid: alpha-ketoglutarate aminotransferases.
June 1984, Life sciences,
Kaan Koper, and Shogo Hataya, and Andrew G Hall, and Taichi E Takasuka, and Hiroshi A Maeda
Activity of aromatic aminotransferases in rat brain.
January 1968, Annales medicinae experimentalis et biologiae Fenniae,
Kaan Koper, and Shogo Hataya, and Andrew G Hall, and Taichi E Takasuka, and Hiroshi A Maeda
Versatility of putative aromatic aminotransferases from Candida albicans.
January 2018, Fungal genetics and biology : FG & B,
Kaan Koper, and Shogo Hataya, and Andrew G Hall, and Taichi E Takasuka, and Hiroshi A Maeda
Biochemical characterization of stage-specific isoforms of aspartate aminotransferases from Trypanosoma cruzi and Trypanosoma brucei.
September 2008, Molecular and biochemical parasitology,
Kaan Koper, and Shogo Hataya, and Andrew G Hall, and Taichi E Takasuka, and Hiroshi A Maeda
The biochemical mechanisms of the plant activation of promutagenic aromatic amines.
January 1990, Environmental and molecular mutagenesis,
Copied contents to your clipboard!