Although numerous ecotoxicological assessments of European honeybee (Apis mellifera L.) have been performed, Japanese wild bees are less well studied in this regard. To address this knowledge gap, we assessed the mortality and acute toxicity (LD50) of 3 common agricultural insecticides (clothianidin, fipronil, and diazinon) on as many as 6 species of Japanese wild bees (Andrena prostomias Perez, Apis cerana japonica Radoszkowski, Bombus deuteronymus Perez, Bombus honshuensis Tkalcu, Bombus hypocrita Perez, and Eucera spp.; all or any of them). The datasets were obtained via standard acute toxicity testing, with assessment of mortality at 24 and 48 h after exposure to the insecticides. These data provide important information regarding the effects of insecticides on Japanese wild bees and their conservation.