-transparent.png){kind=logo}
The binding of antithrombin III, thrombin, thrombin-antithrombin III complex to endothelial cells was investigated. While the rate of the binding of thrombin to these cells was very rapid, that of antithrombin III was relatively slow and the thrombin-antithrombin III complex was intermediate. Binding kinetics indicated that antithrombin III, like thrombin, showed high affinity to endothelial cells; with a Kd of 3 X 10(-8) M and with 5 X 10(4) binding sites per cell. The dissociation of the inhibitor molecule was also rapid, i.e., approximately 70% bound antithrombin III was released in 2 minutes. Heparin, in a 100-fold molar excess to antithrombin III, or the modification of lysine residues of the inhibitor involved in the interaction with heparin, did not influence the association of antithrombin III with endothelial cells. In addition, antithrombin III did not compete with thrombin blocked in its active center for binding to endothelial cells. It is suggested that the binding sites of endothelial cells are different for thrombin and antithrombin III, and antithrombin III does not bind to these cells through its heparin binding domain.
K Bartha, and
T Kovács, and
I Léránt, and
B Papp, and
E Csonka, and
K Kolev, and
R Machovich
April 1988,
Thrombosis research,
K Bartha, and
T Kovács, and
I Léránt, and
B Papp, and
E Csonka, and
K Kolev, and
R Machovich
October 1989,
Thrombosis research,
K Bartha, and
T Kovács, and
I Léránt, and
B Papp, and
E Csonka, and
K Kolev, and
R Machovich
September 1989,
Biochemical and biophysical research communications,
K Bartha, and
T Kovács, and
I Léránt, and
B Papp, and
E Csonka, and
K Kolev, and
R Machovich
August 1989,
Biochimica et biophysica acta,
K Bartha, and
T Kovács, and
I Léránt, and
B Papp, and
E Csonka, and
K Kolev, and
R Machovich
January 1977,
Federation proceedings,
K Bartha, and
T Kovács, and
I Léránt, and
B Papp, and
E Csonka, and
K Kolev, and
R Machovich
March 1995,
Nihon rinsho. Japanese journal of clinical medicine,
K Bartha, and
T Kovács, and
I Léránt, and
B Papp, and
E Csonka, and
K Kolev, and
R Machovich
January 2017,
Journal of orthopaedic surgery (Hong Kong),
K Bartha, and
T Kovács, and
I Léránt, and
B Papp, and
E Csonka, and
K Kolev, and
R Machovich
September 1999,
Nihon rinsho. Japanese journal of clinical medicine,
K Bartha, and
T Kovács, and
I Léránt, and
B Papp, and
E Csonka, and
K Kolev, and
R Machovich
June 1991,
Thrombosis research,
K Bartha, and
T Kovács, and
I Léránt, and
B Papp, and
E Csonka, and
K Kolev, and
R Machovich
October 1991,
Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis,
