BIOMAT Database Logo BIOMAT Database Logo

Protopanaxadiol ameliorates NAFLD by regulating hepatocyte lipid metabolism through AMPK/SIRT1 signaling pathway. 2023

Yiping Li, and Yang Liu, and Zhiwei Chen, and Kaiyue Tang, and Lili Yang, and Yuwei Jiang, and Jue Wang, and Ping Huang, and Jianyi Wang, and Peiyong Zheng, and Haiyan Song
Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China; Collaborative Innovation Center for Biomedicine, Shanghai University of Medicine & Health Sciences, Shanghai 201318, China.

Non-alcoholic fatty liver disease (NAFLD) has become one of the main chronic liver diseases worldwide. Protopanaxadiol (PPD), an active compound derived from Gynostemma pentaphyllum, has been found able to improve free fatty acid-induced lipid accumulation in hepatocytes. However, the efficacy of PPD on NAFLD and the underlying mechanism remains unknown. In this study, the mice were fed with a high-fat diet for 22 weeks to induce the NAFLD model, and then were treated with PPD by gavage for 8 weeks. Moreover, AML12 and HepG2 cells induced by free fatty acids for 24 h, were treated with different doses of PPD and/or AMPK or SIRT1 inhibitor to explore the pharmacological mechanism of PPD. The results showed that mice with PPD treatment had significantly reduced liver weight and serum aminotransferase levels, less severe hepatosteatosis, and inflammatory cell infiltration in liver tissues when compared with the model mice. PPD also reversed the down-regulated activation of AMPK and SIRT1 expression as well as the change of lipid metabolism-related molecules in the mice liver tissues. Consistently, the in vitro experiments showed the effect of PPD in ameliorating lipid accumulation in hepatocytes. The inhibitor of AMPK or SIRT1 suppressed the AMPK and SIRT1 signaling and markedly diminished the anti-steatosis effect of PPD. In conclusion, our results prove the ameliorating impact of PPD on NAFLD and also reveal the involvement of regulation of AMPK/SIRT1 signaling pathway-mediated lipid metabolism in the underlying mechanism, suggesting PPD as a potential natural compound for the treatment of NAFLD.

UI MeSH Term Description Entries
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D005230 Fatty Acids, Nonesterified FATTY ACIDS found in the plasma that are complexed with SERUM ALBUMIN for transport. These fatty acids are not in glycerol ester form. Fatty Acids, Free,Free Fatty Acid,Free Fatty Acids,NEFA,Acid, Free Fatty,Acids, Free Fatty,Acids, Nonesterified Fatty,Fatty Acid, Free,Nonesterified Fatty Acids
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015398 Signal Transduction The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway. Cell Signaling,Receptor-Mediated Signal Transduction,Signal Pathways,Receptor Mediated Signal Transduction,Signal Transduction Pathways,Signal Transduction Systems,Pathway, Signal,Pathway, Signal Transduction,Pathways, Signal,Pathways, Signal Transduction,Receptor-Mediated Signal Transductions,Signal Pathway,Signal Transduction Pathway,Signal Transduction System,Signal Transduction, Receptor-Mediated,Signal Transductions,Signal Transductions, Receptor-Mediated,System, Signal Transduction,Systems, Signal Transduction,Transduction, Signal,Transductions, Signal
D050356 Lipid Metabolism Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS. Metabolism, Lipid
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D055372 AMP-Activated Protein Kinases Intracellular signaling protein kinases that play a signaling role in the regulation of cellular energy metabolism. Their activity largely depends upon the concentration of cellular AMP which is increased under conditions of low energy or metabolic stress. AMP-activated protein kinases modify enzymes involved in LIPID METABOLISM, which in turn provide substrates needed to convert AMP into ATP. 5'-AMP-Activated Protein Kinase,AMP-Activated Kinase,AMP-Activated Protein Kinase,AMP-Activated Protein Kinase alpha Subunit,AMP-Activated Protein Kinase alpha Subunits,AMP-Activated Protein Kinase beta Subunit,AMP-Activated Protein Kinase beta Subunits,AMP-Activated Protein Kinase gamma Subunit,AMP-Activated Protein Kinase gamma Subunits,PRKAA,5' AMP Activated Protein Kinase,AMP Activated Kinase,AMP Activated Protein Kinase,AMP Activated Protein Kinase alpha Subunit,AMP Activated Protein Kinase alpha Subunits,AMP Activated Protein Kinase beta Subunit,AMP Activated Protein Kinase beta Subunits,AMP Activated Protein Kinase gamma Subunit,AMP Activated Protein Kinase gamma Subunits,AMP Activated Protein Kinases
D056564 Sirtuin 1 A sirtuin family member found primarily in the CELL NUCLEUS. It is an NAD-dependent deacetylase with specificity towards HISTONES and a variety of proteins involved in gene regulation. Silent Mating Type Information Regulation 2 Homolog 1,Sirt1
D059305 Diet, High-Fat Consumption of excessive DIETARY FATS. Diet, High Fat,Diets, High Fat,Diets, High-Fat,High Fat Diet,High Fat Diets,High-Fat Diet,High-Fat Diets

Related Publications

Yiping Li, and Yang Liu, and Zhiwei Chen, and Kaiyue Tang, and Lili Yang, and Yuwei Jiang, and Jue Wang, and Ping Huang, and Jianyi Wang, and Peiyong Zheng, and Haiyan Song
Dioscin alleviates non-alcoholic fatty liver disease through adjusting lipid metabolism via SIRT1/AMPK signaling pathway.
May 2018, Pharmacological research,
Yiping Li, and Yang Liu, and Zhiwei Chen, and Kaiyue Tang, and Lili Yang, and Yuwei Jiang, and Jue Wang, and Ping Huang, and Jianyi Wang, and Peiyong Zheng, and Haiyan Song
Monacolin K affects lipid metabolism through SIRT1/AMPK pathway in HepG2 cells.
December 2013, Archives of pharmacal research,
Yiping Li, and Yang Liu, and Zhiwei Chen, and Kaiyue Tang, and Lili Yang, and Yuwei Jiang, and Jue Wang, and Ping Huang, and Jianyi Wang, and Peiyong Zheng, and Haiyan Song
Neferine ameliorates nonalcoholic steatohepatitis through regulating AMPK pathway.
June 2023, Phytomedicine : international journal of phytotherapy and phytopharmacology,
Yiping Li, and Yang Liu, and Zhiwei Chen, and Kaiyue Tang, and Lili Yang, and Yuwei Jiang, and Jue Wang, and Ping Huang, and Jianyi Wang, and Peiyong Zheng, and Haiyan Song
Extract of Silphium perfoliatum L. improve lipid accumulation in NAFLD mice by regulating AMPK/FXR signaling pathway.
June 2024, Journal of ethnopharmacology,
Yiping Li, and Yang Liu, and Zhiwei Chen, and Kaiyue Tang, and Lili Yang, and Yuwei Jiang, and Jue Wang, and Ping Huang, and Jianyi Wang, and Peiyong Zheng, and Haiyan Song
Salidroside ameliorates diabetic nephropathy in rats by activating renal AMPK/SIRT1 signaling pathway.
April 2020, Journal of food biochemistry,
Yiping Li, and Yang Liu, and Zhiwei Chen, and Kaiyue Tang, and Lili Yang, and Yuwei Jiang, and Jue Wang, and Ping Huang, and Jianyi Wang, and Peiyong Zheng, and Haiyan Song
AMPK regulates energy metabolism through the SIRT1 signaling pathway to improve myocardial hypertrophy.
May 2018, European review for medical and pharmacological sciences,
Yiping Li, and Yang Liu, and Zhiwei Chen, and Kaiyue Tang, and Lili Yang, and Yuwei Jiang, and Jue Wang, and Ping Huang, and Jianyi Wang, and Peiyong Zheng, and Haiyan Song
Ezetimibe ameliorates lipid accumulation during adipogenesis by regulating the AMPK-mTORC1 pathway.
January 2020, FASEB journal : official publication of the Federation of American Societies for Experimental Biology,
Yiping Li, and Yang Liu, and Zhiwei Chen, and Kaiyue Tang, and Lili Yang, and Yuwei Jiang, and Jue Wang, and Ping Huang, and Jianyi Wang, and Peiyong Zheng, and Haiyan Song
Rap1GAP exacerbates myocardial infarction by regulating the AMPK/SIRT1/NF-κB signaling pathway.
May 2024, Cellular signalling,
Yiping Li, and Yang Liu, and Zhiwei Chen, and Kaiyue Tang, and Lili Yang, and Yuwei Jiang, and Jue Wang, and Ping Huang, and Jianyi Wang, and Peiyong Zheng, and Haiyan Song
APPL1 ameliorates myocardial ischemia-reperfusion injury by regulating the AMPK signaling pathway.
February 2022, Experimental and therapeutic medicine,
Yiping Li, and Yang Liu, and Zhiwei Chen, and Kaiyue Tang, and Lili Yang, and Yuwei Jiang, and Jue Wang, and Ping Huang, and Jianyi Wang, and Peiyong Zheng, and Haiyan Song
Colchicine ameliorates myocardial injury induced by coronary microembolization through suppressing pyroptosis via the AMPK/SIRT1/NLRP3 signaling pathway.
January 2024, BMC cardiovascular disorders,
Copied contents to your clipboard!