Maternal exposure to a glyphosate-based herbicide impairs placental development through endoplasmic reticulum stress in mice. 2023

Mengling Liu, and Siying Lu, and Chuanzhen Yang, and Dalei Zhang, and Jun Zhu, and Jiting Yin, and Hongru Zhao, and Bei Yang, and Haibin Kuang
Department of Physiology, Basic Medical College, Nanchang University, Nanchang, Jiangxi, 330006, PR China; Nursing School of Jiujiang University, Jiujiang, Jiangxi, 332000, PR China. Electronic address: 304916968@qq.com.

Glyphosate-based herbicides (GBHs) are the most widely used agrochemicals worldwide, increasing the risk of their occurrence in the environment. This study aimed to explore effects and mechanisms of GBH exposure on placental development in vivo during pregnancy in mice. Pregnant mice received GBH by gavage at 0, 5, and 50 mg⋅kg-1⋅day-1 doses from gestational day (GD) 1 to GD 13 and were sacrificed on GD 13 or GD19. Our data indicated that GBH administration significantly increased the number of resorbed fetuses, reduced the weight of fetuses and placentas, and inhibited placental growth, as evident from decreased placental total area and spongiotrophoblast area on GD 19. GBH treatment also inhibited proliferation and induced apoptosis of placenta via upregulation of Bax, cleaved caspase-3 and -12 expression, and downregulation of B cell lymphoma (Bcl)-2 expression. Further study showed that GBH exposure significantly increased expression levels of glucose-regulated protein 78 (GRP78), protein kinase RNA-like endoplasmic reticulum kinase (PERK), and C/EBP homologous protein (CHOP) mRNAs and proteins and triggered oxidative stress in placenta on GD 13 and GD 19. In conclusion, our findings suggest that maternal exposure to GBH can impair placental development through the endoplasmic reticulum stress-mediated activation of GRP78/PERK/CHOP signaling pathway in mice.

UI MeSH Term Description Entries
D010920 Placenta A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES). Placentoma, Normal,Placentome,Placentas,Placentomes
D010929 Placentation The development of the PLACENTA, a highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products between mother and FETUS. The process begins at FERTILIZATION, through the development of CYTOTROPHOBLASTS and SYNCYTIOTROPHOBLASTS, the formation of CHORIONIC VILLI, to the progressive increase in BLOOD VESSELS to support the growing fetus. Hemochorial Placental Development,Hemochorial Placentation,Placental Development,Placental Development, Hemochorial,Placentation, Hemochorial
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D005260 Female Females
D006540 Herbicides Pesticides used to destroy unwanted vegetation, especially various types of weeds, grasses (POACEAE), and woody plants. Some plants develop HERBICIDE RESISTANCE. Algaecide,Algicide,Herbicide,Algaecides,Algicides
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000091342 Endoplasmic Reticulum Chaperone BiP An ENDOPLASMIC RETICULUM specific chaperone of the HSP70 family. They are involved in folding and oligomerization of secreted and membrane proteins and ENDOPLASMIC RETICULUM STRESS related UNFOLDED PROTEIN RESPONSE. Binding-immunoglobulin Protein Molecular Chaperone,Glucose Regulated Protein 78 kDa,Grp78,HSPA5 Protein,Heat-Shock Protein 5,Molecular Chaperone BiP,Molecular Chaperone GRP78,BiP, Molecular Chaperone,Binding immunoglobulin Protein Molecular Chaperone,GRP78, Molecular Chaperone,Heat Shock Protein 5,Protein, HSPA5
D000097797 Glyphosate Active compound in herbicidal formulations that inhibits 3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE. Gliphosate,Glyphosate Hydrochloride (2:1),Glyphosate, Calcium Salt,Glyphosate, Calcium Salt (1:1),Glyphosate, Copper (2+) Salt,Glyphosate, Dilithium Salt,Glyphosate, Disodium Salt,Glyphosate, Magnesium Salt,Glyphosate, Magnesium Salt (2:1),Glyphosate, Monoammonium Salt,Glyphosate, Monopotassium Salt,Glyphosate, Monosodium Salt,Glyphosate, Sodium Salt,Glyphosate, Zinc Salt,Yerbimat,Kalach 360 SL,N-(phosphonomethyl)glycine,Roundup
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D017209 Apoptosis A regulated cell death mechanism characterized by distinctive morphologic changes in the nucleus and cytoplasm, including the endonucleolytic cleavage of genomic DNA, at regularly spaced, internucleosomal sites, i.e., DNA FRAGMENTATION. It is genetically programmed and serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. Apoptosis, Extrinsic Pathway,Apoptosis, Intrinsic Pathway,Caspase-Dependent Apoptosis,Classic Apoptosis,Classical Apoptosis,Programmed Cell Death,Programmed Cell Death, Type I,Apoptoses, Extrinsic Pathway,Apoptoses, Intrinsic Pathway,Apoptosis, Caspase-Dependent,Apoptosis, Classic,Apoptosis, Classical,Caspase Dependent Apoptosis,Cell Death, Programmed,Classic Apoptoses,Extrinsic Pathway Apoptoses,Extrinsic Pathway Apoptosis,Intrinsic Pathway Apoptoses,Intrinsic Pathway Apoptosis

Related Publications

Mengling Liu, and Siying Lu, and Chuanzhen Yang, and Dalei Zhang, and Jun Zhu, and Jiting Yin, and Hongru Zhao, and Bei Yang, and Haibin Kuang
November 2022, Toxics,
Mengling Liu, and Siying Lu, and Chuanzhen Yang, and Dalei Zhang, and Jun Zhu, and Jiting Yin, and Hongru Zhao, and Bei Yang, and Haibin Kuang
May 2019, Toxicological sciences : an official journal of the Society of Toxicology,
Mengling Liu, and Siying Lu, and Chuanzhen Yang, and Dalei Zhang, and Jun Zhu, and Jiting Yin, and Hongru Zhao, and Bei Yang, and Haibin Kuang
May 2024, Environmental toxicology,
Mengling Liu, and Siying Lu, and Chuanzhen Yang, and Dalei Zhang, and Jun Zhu, and Jiting Yin, and Hongru Zhao, and Bei Yang, and Haibin Kuang
January 2023, The Science of the total environment,
Mengling Liu, and Siying Lu, and Chuanzhen Yang, and Dalei Zhang, and Jun Zhu, and Jiting Yin, and Hongru Zhao, and Bei Yang, and Haibin Kuang
November 2022, Anatomical record (Hoboken, N.J. : 2007),
Mengling Liu, and Siying Lu, and Chuanzhen Yang, and Dalei Zhang, and Jun Zhu, and Jiting Yin, and Hongru Zhao, and Bei Yang, and Haibin Kuang
February 2024, Toxicology and applied pharmacology,
Mengling Liu, and Siying Lu, and Chuanzhen Yang, and Dalei Zhang, and Jun Zhu, and Jiting Yin, and Hongru Zhao, and Bei Yang, and Haibin Kuang
January 2017, Internal medicine (Tokyo, Japan),
Mengling Liu, and Siying Lu, and Chuanzhen Yang, and Dalei Zhang, and Jun Zhu, and Jiting Yin, and Hongru Zhao, and Bei Yang, and Haibin Kuang
March 2023, The Science of the total environment,
Mengling Liu, and Siying Lu, and Chuanzhen Yang, and Dalei Zhang, and Jun Zhu, and Jiting Yin, and Hongru Zhao, and Bei Yang, and Haibin Kuang
March 2024, Naunyn-Schmiedeberg's archives of pharmacology,
Mengling Liu, and Siying Lu, and Chuanzhen Yang, and Dalei Zhang, and Jun Zhu, and Jiting Yin, and Hongru Zhao, and Bei Yang, and Haibin Kuang
September 2021, Environmental pollution (Barking, Essex : 1987),
Copied contents to your clipboard!