Biomaterial Database

[Simple method of embedding in paraffin under vacuum]. 1978

L K Krivonosov

A method of embedding into paraffin under vacuum is proposed. Pieces of tissues are placed into a glass jar containing melted paraffin from which the air is pumped out. The jar is permanently placed into TC-80 thermostate. The air-removing hose is placed inside the thermostate through the opening in its upper wall meant for the control thermometer. The air is pumped out by any vacuum pump with electric drive. The proposed method is simple, provides for a good saturation with paraffin, shortens the time of tissue contact with melted paraffin, and gives thin sections of good quality.

UI	MeSH Term	Description	Entries
D010232	Paraffin	A mixture of solid hydrocarbons obtained from petroleum. It has a wide range of uses including as a stiffening agent in ointments, as a lubricant, and as a topical anti-inflammatory. It is also commonly used as an embedding material in histology.	Parafilm
D003584	Cytological Techniques	Methods used to study CELLS.	Cytologic Technics,Cytological Technic,Cytological Technics,Cytological Technique,Technic, Cytological,Technics, Cytological,Technique, Cytological,Techniques, Cytological,Cytologic Technic,Technic, Cytologic,Technics, Cytologic
D014618	Vacuum	A space in which the pressure is far below atmospheric pressure so that the remaining gases do not affect processes being carried on in the space.	Vacuums