Biomaterial Database

Evaluation of two vacuum bottle blood culture media--supplemented peptone broth and brain heart infusion broth. 1987

P Sandven, and E A Høiby, and L Vorland

National Institute of Public Health, Oslo, Norway.

A new blood culture medium (brain heart infusion broth) (BHI) from Terumo was compared with a commonly used blood culture medium, supplemented peptone broth (Becton Dickinson) (SPB). Blood from patients with suspected bacteraemia was simultaneously cultured in the two media, approximately five ml of blood in each bottle. Both bottles were incubated aerobically at 37 degrees C for 7 days. A total of 3724 paired blood cultures (sets) were processed. Altogether 340 (9.1%) of the blood culture sets were positive. Of these, 233 were positive in both media, 51 in the SPB medium only and 56 in the BHI medium only. Despite the similar overall results, Streptococcus spp. and anaerobic bacteria were detected more frequently in the SPB bottles and Enterobacteriaceae more frequently in the BHI bottles.

UI	MeSH Term	Description	Entries
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D004755	Enterobacteriaceae	A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock.	Coliform Bacilli,Enterobacteria,Ewingella,Leclercia,Paracolobactrum,Sodalis
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001421	Bacteria, Anaerobic	Bacteria that can survive and grow in the complete, or nearly complete absence of oxygen.	Anaerobic Bacteria
D013291	Streptococcus	A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.
D018805	Sepsis	Systemic inflammatory response syndrome with a proven or suspected infectious etiology. When sepsis is associated with organ dysfunction distant from the site of infection, it is called severe sepsis. When sepsis is accompanied by HYPOTENSION despite adequate fluid infusion, it is called SEPTIC SHOCK.	Bloodstream Infection,Pyaemia,Pyemia,Pyohemia,Blood Poisoning,Poisoning, Blood,Septicemia,Severe Sepsis,Blood Poisonings,Bloodstream Infections,Infection, Bloodstream,Poisonings, Blood,Pyaemias,Pyemias,Pyohemias,Sepsis, Severe,Septicemias