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A chromatographic method based on "split-peak" behavior was described for the determination of the coefficient of mass transfer of proteins on small reversed-phase columns. The coefficient of mass transfer was found to be a linear function of the protein translational diffusion coefficient and inversely proportional to the square of the support particle diameter, as predicted by chromatographic theory. As an example of the practical application of this method for the measurement of protein diffusion coefficients, the denaturation of bovine serum albumin with decreasing solution pH was followed by measuring the change in the coefficient of mass transfer. A major advantage of this method was that the results were not affected by the interaction of the protein with the stationary phase.
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March 1984,
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January 1994,
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R R Walters
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L A Larew, and
R R Walters
June 1962,
Science (New York, N.Y.),
L A Larew, and
R R Walters
January 1985,
Revista de chirurgie, oncologie, radiologie, o. r. l., oftalmologie, stomatologie. Seria: Stomatologie,
L A Larew, and
R R Walters
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L A Larew, and
R R Walters
September 1968,
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L A Larew, and
R R Walters
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L A Larew, and
R R Walters
November 1966,
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L A Larew, and
R R Walters
January 2019,
Methods in molecular biology (Clifton, N.J.),
