Polyhydroxyalkanoates and 5-aminolevulinic acid production by a mixed phototrophic culture using medium-chain carboxylic acids from winery effluents. 2023

Virginia Montiel-Corona, and Germán Buitrón
Laboratory for Research on Advanced Processes for Water Treatment, Unidad Académica Juriquilla, Instituto de Ingeniería, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro 76230, México.

This work aimed to obtain polyhydroxyalkanoates (PHA) and 5-aminolevulinic acid (5-ALA) from medium-chain carboxylic acids (MCCA) using a mixed culture enriched in Rhodopseudomnas palustris. MCCA, obtained from residual wine lees, were tested in batch photofermentation experiments. First, the influence of individual MCCA (hexanoic, heptanoic, and octanoic acids) was evaluated; then, the MCCA coming directly from a fermentation reactor (LC-effluent) or after acids extraction (HC-effluent) were studied. Nutrient supplementation, bicarbonate, and acetic acid addition were also tested. Results showed that PHA production was higher in hexanoic (328 mg PHA/L) compared to heptanoic (152 mg PHA/L) and octanoic (164 mg PHA/L) acids. Bicarbonate addition and acetic acid as co-substrate improved the MCCA consumption, the PHA content and production rate. The HC-effluent, without nutrient supplementation, was allowed to increase 2.5 times the PHA content (reaching 40 % w/w and 584 mg/L) and to double 5-ALA production (7.6 µM) compared to the LC-effluent condition.

UI MeSH Term Description Entries
D002264 Carboxylic Acids Organic compounds containing the carboxy group (-COOH). This group of compounds includes amino acids and fatty acids. Carboxylic acids can be saturated, unsaturated, or aromatic. Carboxylic Acid,Acid, Carboxylic,Acids, Carboxylic
D005285 Fermentation Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID. Fermentations
D000622 Aminolevulinic Acid A compound produced from succinyl-CoA and GLYCINE as an intermediate in heme synthesis. It is used as a PHOTOCHEMOTHERAPY for actinic KERATOSIS. 5-Amino Levulinic Acid,5-Aminolaevulinate,5-Aminolevulinate,Aminolevulinic Acid Hydrochloride,Delta-Aminolevulinic Acid,Levulan,5 Amino Levulinic Acid,5 Aminolaevulinate,5 Aminolevulinate,Acid Hydrochloride, Aminolevulinic,Acid, 5-Amino Levulinic,Acid, Aminolevulinic,Acid, Delta-Aminolevulinic,Delta Aminolevulinic Acid,Hydrochloride, Aminolevulinic Acid,Levulinic Acid, 5-Amino
D001639 Bicarbonates Inorganic salts that contain the -HCO3 radical. They are an important factor in determining the pH of the blood and the concentration of bicarbonate ions is regulated by the kidney. Levels in the blood are an index of the alkali reserve or buffering capacity. Bicarbonate,Bicarbonate Ions,Hydrogen Carbonates,Bicarbonate Ion,Carbonic Acid Ions,Hydrogen Carbonate,Carbonate, Hydrogen,Carbonates, Hydrogen,Ion, Bicarbonate,Ions, Bicarbonate,Ions, Carbonic Acid
D054813 Polyhydroxyalkanoates Biodegradable microbial polyesters which contain hydroxyacyl monomers. Polyhydroxyalkanoates are carbon and energy reserve materials produced and sequestered in cytoplasmic granules called polyhydroxyalkanoate or PHA granules by various archaea and bacteria and may additionally provide protective mechanism to the microbes. They are biosynthesized by microbial polyhydroxyalkanoate synthase enzymes using fatty acids or citramalate synthase enzymes using glucose. Poly(hydroxyalkanoate),Poly(hydroxyalkanoates),Polyhydroxyalkanoate
D019149 Bioreactors Tools or devices for generating products using the synthetic or chemical conversion capacity of a biological system. They can be classical fermentors, cell culture perfusion systems, or enzyme bioreactors. For production of proteins or enzymes, recombinant microorganisms such as bacteria, mammalian cells, or insect or plant cells are usually chosen. Fermentors,Bioreactor,Fermentor
D019342 Acetic Acid Product of the oxidation of ethanol and of the destructive distillation of wood. It is used locally, occasionally internally, as a counterirritant and also as a reagent. (Stedman, 26th ed) Glacial Acetic Acid,Vinegar,Acetic Acid Glacial,Acetic Acid, Glacial,Glacial, Acetic Acid

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