BACKGROUND Scatter radiation is a traditional problem in cone-beam computed tomography (CBCT). Accordingly, numerous methods have been researched for scatter reduction in terms of software- or hardware-based solutions. The concept of a two-dimensional antiscatter grid (2D-ASG) has been shown to provide a solution to the scatter reduction in CBCT. However, the use of an ASG makes it challenging to use in clinical CBCT systems because it causes Moire artifacts of the image. OBJECTIVE We have developed a Moire-free 2D-ASG that was designed to solve the Moire artifact and the scatter radiation problems. We provide the experimental results pertaining to the image quality measurements from the evaluation of the 2D-ASG compared with those obtained from a one-dimensional antiscatter grid (1D-ASG) and no antiscatter grid (No-ASG) to demonstrate the quantitative extent of the improvements. METHODS The 2D-ASG, fabricated based on a sawing process with a graphite body, was prepared to evaluate image quality improvements. Projection images for Pro-CT MK II phantom were acquired using the CBCT testbed of sample rotation type and reconstructed by Feldkamp-Davis-Kress (FDK) algorithm without any filters. We measured the contrast-to-noise ratio (CNR) and uniformity index for the cupping artifacts of the 2D-ASG, 1D-ASG, and No-ASG cases. RESULTS 2D-ASG considerably reduced the cupping artifacts owing to the scatter reduction compared with the 1D-ASG and No-ASG cases. The cupping artifacts were reduced by 85% in the 2D-ASG compared with No-ASG, while the cupping artifacts were reduced by 63% in 1D-ASG compared with No-ASG. The 2D-ASG also yielded a CNR improvement. The average CNR improvements for eight insert materials were 47% in the 2D-ASG compared with No-ASG, while the CNR was improved by 36% in the 1D-ASG compared with No-ASG. CONCLUSIONS We demonstrated that the Moire-free 2D-ASG improved the image quality by removing scatter radiation in CBCT compared with 1D-ASG and No-ASG. We believe that the Moire-free 2D-ASG can become one of the effective ways to solve the scatter radiation problem in CBCT images because it provides usability and has the potential to have synergistic effects on other methods, such as bow-tie filter and scatter correction.