BIOMAT Database Logo BIOMAT Database Logo

Structure of pyridoxal 5'-phosphate-bound D-threonine aldolase from Chlamydomonas reinhardtii. 2023

Yuki Hirato, and Masaru Goto, and Taichi Mizobuchi, and Hisashi Muramatsu, and Minoru Tanigawa, and Katsushi Nishimura
Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan.

D-Threonine aldolase (DTA) is a pyridoxal-5'-phosphate-dependent enzyme which catalyzes the reversible aldol reaction of glycine with a corresponding aldehyde to yield the D-form β-hydroxy-α-amino acid. This study produced and investigated the crystal structure of DTA from Chlamydomonas reinhardtii (CrDTA) at 1.85 Å resolution. To our knowledge, this is the first report on the crystal structure of eukaryotic DTA. Compared with the structure of bacterial DTA, CrDTA has a similar arrangement of active-site residues. On the other hand, we speculated that some non-conserved residues alter the affinity for substrates and inhibitors. The structure of CrDTA could provide insights into the structural framework for structure-guided protein engineering studies to modify reaction selectivity.

UI MeSH Term Description Entries
D010710 Phosphates Inorganic salts of phosphoric acid. Inorganic Phosphate,Phosphates, Inorganic,Inorganic Phosphates,Orthophosphate,Phosphate,Phosphate, Inorganic
D011732 Pyridoxal Phosphate This is the active form of VITAMIN B 6 serving as a coenzyme for synthesis of amino acids, neurotransmitters (serotonin, norepinephrine), sphingolipids, aminolevulinic acid. During transamination of amino acids, pyridoxal phosphate is transiently converted into pyridoxamine phosphate (PYRIDOXAMINE). Pyridoxal 5-Phosphate,Pyridoxal-P,Phosphate, Pyridoxal,Pyridoxal 5 Phosphate,Pyridoxal P
D012696 Glycine Hydroxymethyltransferase A pyridoxal phosphate enzyme that catalyzes the reaction of glycine and 5,10-methylene-tetrahydrofolate to form serine. It also catalyzes the reaction of glycine with acetaldehyde to form L-threonine. EC 2.1.2.1. Serine Aldolase,Serine Hydroxymethylase,Serine Hydroxymethyltransferase,Serine Transhydroxymethylase,Threonine Aldolase,Allothreonine Aldolase,Aldolase, Allothreonine,Aldolase, Serine,Aldolase, Threonine,Hydroxymethylase, Serine,Hydroxymethyltransferase, Glycine,Hydroxymethyltransferase, Serine,Transhydroxymethylase, Serine
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D016825 Chlamydomonas reinhardtii A species of GREEN ALGAE. Delicate, hairlike appendages arise from the flagellar surface in these organisms. Chlamydomonas reinhardii,Chlamydomonas reinhardius,Chlamydomonas reinhardtius,reinhardius, Chlamydomonas,reinhardtii, Chlamydomonas
D018360 Crystallography, X-Ray The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) X-Ray Crystallography,Crystallography, X Ray,Crystallography, Xray,X Ray Crystallography,Xray Crystallography,Crystallographies, X Ray,X Ray Crystallographies

Related Publications

Yuki Hirato, and Masaru Goto, and Taichi Mizobuchi, and Hisashi Muramatsu, and Minoru Tanigawa, and Katsushi Nishimura
Cloning and characterization of d-threonine aldolase from the green alga Chlamydomonas reinhardtii.
March 2017, Phytochemistry,
Yuki Hirato, and Masaru Goto, and Taichi Mizobuchi, and Hisashi Muramatsu, and Minoru Tanigawa, and Katsushi Nishimura
Isolation and characterization of D-threonine aldolase, a pyridoxal-5'-phosphate-dependent enzyme from Arthrobacter sp. DK-38.
September 1997, European journal of biochemistry,
Yuki Hirato, and Masaru Goto, and Taichi Mizobuchi, and Hisashi Muramatsu, and Minoru Tanigawa, and Katsushi Nishimura
Structure of alanine racemase from Oenococcus oeni with bound pyridoxal 5'-phosphate.
January 2013, Acta crystallographica. Section F, Structural biology and crystallization communications,
Yuki Hirato, and Masaru Goto, and Taichi Mizobuchi, and Hisashi Muramatsu, and Minoru Tanigawa, and Katsushi Nishimura
D-glucosaminate dehydratase: spectrometric properties of the enzyme-bound pyridoxal 5'-phosphate.
January 1984, Journal of biochemistry,
Yuki Hirato, and Masaru Goto, and Taichi Mizobuchi, and Hisashi Muramatsu, and Minoru Tanigawa, and Katsushi Nishimura
Crystal structure of chloroplast fructose-1,6-bisphosphate aldolase from the green alga Chlamydomonas reinhardtii.
September 2022, Journal of structural biology,
Yuki Hirato, and Masaru Goto, and Taichi Mizobuchi, and Hisashi Muramatsu, and Minoru Tanigawa, and Katsushi Nishimura
The plastid aldolase gene from Chlamydomonas reinhardtii: intron/exon organization, evolution, and promoter structure.
August 1995, Molecular & general genetics : MGG,
Yuki Hirato, and Masaru Goto, and Taichi Mizobuchi, and Hisashi Muramatsu, and Minoru Tanigawa, and Katsushi Nishimura
Structure of GUN4 from Chlamydomonas reinhardtii.
August 2015, Acta crystallographica. Section F, Structural biology communications,
Yuki Hirato, and Masaru Goto, and Taichi Mizobuchi, and Hisashi Muramatsu, and Minoru Tanigawa, and Katsushi Nishimura
Structure and function of the pyridoxal 5'-phosphate-dependent (PLP) threonine deaminase IlvA1 from Pseudomonas aeruginosa PAO1.
April 2024, Biochemical and biophysical research communications,
Yuki Hirato, and Masaru Goto, and Taichi Mizobuchi, and Hisashi Muramatsu, and Minoru Tanigawa, and Katsushi Nishimura
Structure and control of pyridoxal phosphate dependent allosteric threonine deaminase.
April 1998, Structure (London, England : 1993),
Yuki Hirato, and Masaru Goto, and Taichi Mizobuchi, and Hisashi Muramatsu, and Minoru Tanigawa, and Katsushi Nishimura
Characterization of chloroplast ribulose-5-phosphate-3-epimerase from the microalga Chlamydomonas reinhardtii.
March 2024, Plant physiology,
Copied contents to your clipboard!