BIOMAT Database Logo BIOMAT Database Logo

Characterization and Biodegradation of Phenol by Pseudomonas aeruginosa and Klebsiella variicola Strains Isolated from Sewage Sludge and Their Effect on Soybean Seeds Germination. 2023

Samir A Mahgoub, and Shaza Y A Qattan, and Salma S Salem, and Howaida M Abdelbasit, and Mohamed Raafat, and Mada F Ashkan, and Diana A Al-Quwaie, and Ebtihal Abdullah Motwali, and Fatimah S Alqahtani, and Hassan I Abd El-Fattah
Department of Agricultural Microbiology, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt.

Phenols are very soluble in water; as a result, they can pollute a massive volume of fresh water, wastewater, groundwater, oceans, and soil, negatively affecting plant germination and animal and human health. For the detoxification and bioremediation of phenol in wastewater, phenol biodegradation using novel bacteria isolated from sewage sludge was investigated. Twenty samples from sewage sludge (SS) were collected, and bacteria in SS contents were cultured in the mineral salt agar (MSA) containing phenol (500 mg/L). Twenty colonies (S1 up to S20) were recovered from all the tested SS samples. The characteristics of three bacterial properties, 16S rDNA sequencing, similarities, GenBank accession number, and phylogenetic analysis showed that strains S3, S10, and S18 were Pseudomonas aeruginosa, Klebsiella pneumoniae, and Klebsiella variicola, respectively. P. aeruginosa, K. pneumoniae, and K. variicola were able to degrade 1000 mg/L phenol in the mineral salt medium. The bacterial strains from sewage sludge were efficient in removing 71.70 and 74.67% of phenol at 1000 mg/L within three days and could tolerate high phenol concentrations (2000 mg/L). The findings showed that P. aeruginosa, K. pneumoniae, and K. variicola could potentially treat phenolic water. All soybean and faba bean seeds were germinated after being treated with 250, 500, 750, and 1000 mg/L phenol in a mineral salt medium inoculated with these strains. The highest maximum phenol removal and detoxification rates were P. aeruginosa and K. variicola. These strains may help decompose and detoxify phenol from industrial wastewater with high phenol levels and bioremediating phenol-contaminated soils.

UI MeSH Term Description Entries
D007711 Klebsiella pneumoniae Gram-negative, non-motile, capsulated, gas-producing rods found widely in nature and associated with urinary and respiratory infections in humans. Bacillus pneumoniae,Bacterium pneumoniae crouposae,Hyalococcus pneumoniae,Klebsiella pneumoniae aerogenes,Klebsiella rhinoscleromatis
D008903 Minerals Native, inorganic or fossilized organic substances having a definite chemical composition and formed by inorganic reactions. They may occur as individual crystals or may be disseminated in some other mineral or rock. (Grant & Hackh's Chemical Dictionary, 5th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Mineral
D010636 Phenols Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.
D010802 Phylogeny The relationships of groups of organisms as reflected by their genetic makeup. Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D011550 Pseudomonas aeruginosa A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection. Bacillus aeruginosus,Bacillus pyocyaneus,Bacterium aeruginosum,Bacterium pyocyaneum,Micrococcus pyocyaneus,Pseudomonas polycolor,Pseudomonas pyocyanea
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001419 Bacteria One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive. Eubacteria
D001673 Biodegradation, Environmental Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers. Bioremediation,Phytoremediation,Natural Attenuation, Pollution,Environmental Biodegradation,Pollution Natural Attenuation
D012639 Seeds The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield. Diaspores,Elaiosomes,Embryos, Plant,Plant Embryos,Plant Zygotes,Zygotes, Plant,Diaspore,Elaiosome,Embryo, Plant,Plant Embryo,Plant Zygote,Seed,Zygote, Plant
D012722 Sewage Refuse liquid or waste matter carried off by sewers. Sludge,Sludge Flocs

Related Publications

Samir A Mahgoub, and Shaza Y A Qattan, and Salma S Salem, and Howaida M Abdelbasit, and Mohamed Raafat, and Mada F Ashkan, and Diana A Al-Quwaie, and Ebtihal Abdullah Motwali, and Fatimah S Alqahtani, and Hassan I Abd El-Fattah
Biodegradation of pyrene by a Pseudomonas aeruginosa strain RS1 isolated from refinery sludge.
August 2014, Bioresource technology,
Samir A Mahgoub, and Shaza Y A Qattan, and Salma S Salem, and Howaida M Abdelbasit, and Mohamed Raafat, and Mada F Ashkan, and Diana A Al-Quwaie, and Ebtihal Abdullah Motwali, and Fatimah S Alqahtani, and Hassan I Abd El-Fattah
Biodegradation of clopidogrel bisulfate by Pseudomonas aeruginosa and Pseudomonas putida strains isolated from Algerian wastewater.
May 2023, Journal of contaminant hydrology,
Samir A Mahgoub, and Shaza Y A Qattan, and Salma S Salem, and Howaida M Abdelbasit, and Mohamed Raafat, and Mada F Ashkan, and Diana A Al-Quwaie, and Ebtihal Abdullah Motwali, and Fatimah S Alqahtani, and Hassan I Abd El-Fattah
Biodegradation of cefalexin by two bacteria strains from sewage sludge.
January 2023, Royal Society open science,
Samir A Mahgoub, and Shaza Y A Qattan, and Salma S Salem, and Howaida M Abdelbasit, and Mohamed Raafat, and Mada F Ashkan, and Diana A Al-Quwaie, and Ebtihal Abdullah Motwali, and Fatimah S Alqahtani, and Hassan I Abd El-Fattah
Characterization of phenol biodegradation by Comamonas testosteroni ZD4-1 and Pseudomonas aeruginosa ZD4-3.
June 2003, Biomedical and environmental sciences : BES,
Samir A Mahgoub, and Shaza Y A Qattan, and Salma S Salem, and Howaida M Abdelbasit, and Mohamed Raafat, and Mada F Ashkan, and Diana A Al-Quwaie, and Ebtihal Abdullah Motwali, and Fatimah S Alqahtani, and Hassan I Abd El-Fattah
[Toxin production by pseudomonas aeruginosa strains isolated from children and their environment].
January 1977, Medycyna doswiadczalna i mikrobiologia,
Samir A Mahgoub, and Shaza Y A Qattan, and Salma S Salem, and Howaida M Abdelbasit, and Mohamed Raafat, and Mada F Ashkan, and Diana A Al-Quwaie, and Ebtihal Abdullah Motwali, and Fatimah S Alqahtani, and Hassan I Abd El-Fattah
Decolorization and biodegradation of reactive Red 198 Azo dye by a new Enterococcus faecalis-Klebsiella variicola bacterial consortium isolated from textile wastewater sludge.
February 2019, World journal of microbiology & biotechnology,
Samir A Mahgoub, and Shaza Y A Qattan, and Salma S Salem, and Howaida M Abdelbasit, and Mohamed Raafat, and Mada F Ashkan, and Diana A Al-Quwaie, and Ebtihal Abdullah Motwali, and Fatimah S Alqahtani, and Hassan I Abd El-Fattah
Isolation and Characterization of Lytic Pseudomonas aeruginosa Bacteriophages Isolated from Sewage Samples from Tunisia.
October 2022, Viruses,
Samir A Mahgoub, and Shaza Y A Qattan, and Salma S Salem, and Howaida M Abdelbasit, and Mohamed Raafat, and Mada F Ashkan, and Diana A Al-Quwaie, and Ebtihal Abdullah Motwali, and Fatimah S Alqahtani, and Hassan I Abd El-Fattah
Characterization of pyrene degradation by Pseudomonas sp. strain Jpyr-1 isolated from active sewage sludge.
July 2013, Bioresource technology,
Samir A Mahgoub, and Shaza Y A Qattan, and Salma S Salem, and Howaida M Abdelbasit, and Mohamed Raafat, and Mada F Ashkan, and Diana A Al-Quwaie, and Ebtihal Abdullah Motwali, and Fatimah S Alqahtani, and Hassan I Abd El-Fattah
Biodegradation of kraft lignin by newly isolated Klebsiella pneumoniae, Pseudomonas putida, and Ochrobactrum tritici strains.
May 2018, Environmental science and pollution research international,
Samir A Mahgoub, and Shaza Y A Qattan, and Salma S Salem, and Howaida M Abdelbasit, and Mohamed Raafat, and Mada F Ashkan, and Diana A Al-Quwaie, and Ebtihal Abdullah Motwali, and Fatimah S Alqahtani, and Hassan I Abd El-Fattah
Isolation of Novel Bacterial Strains Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 from Sewage Sludge for Paracetamol Biodegradation.
January 2023, Microorganisms,
Copied contents to your clipboard!