-transparent.png){kind=logo}
Establishment and its characteristics of a nude mice solid tumor model NHG-1 from human glioma cell line are reported. 5-8 week old NC nude mice of both sexes and SHG-44 cell line used in this experiment were from our laboratory. The initial successful transplantation rate was 7/11 (64%) and that of 30 passages in the subsequent 4 years was 100%. After subcutaneous inoculation, growth curve showed a latent period in week 1-2, slow growing period in week 3-4, rapid growing period in week 5-6 and a final plateau period in week 7. The doubling time was 7 days and cell cycle time was 2.5 days. The cells in G1, S and G2M phases comprised 56%, 27% and 17%, respectively. The survival time of the host was 54 +/- 15 days. The tumor tissues showed a tendency towards invading the surrounding soft tissues. By morphological observation with light and electron microscopes, LDH isozyme assay, PAP immuno-histochemistry labelling GFAP and chromosome analysis, it is confirmed that the transplantable tumor possesses the characteristics of human malignant glioma. The estrogen receptor in the transplantable tumors demonstrated by cytochemical assay indicates that the glioma carcinogenesis is related to endocrine factor of the host. The therapeutic effects of anticancer drugs, such as ACNU, BCNU and 10-hydroxy-2-decenoic acid from the royal jelly on NHG-1 model are evaluated.
Q Huang, and
Z W Du, and
G D Xu, and
Z Y Liu, and
Y H Guo, and
G L Chen, and
W X Ma, and
Q Y Tan, and
Q N Xu, and
B Li
September 1985,
Zhonghua zhong liu za zhi [Chinese journal of oncology],
Q Huang, and
Z W Du, and
G D Xu, and
Z Y Liu, and
Y H Guo, and
G L Chen, and
W X Ma, and
Q Y Tan, and
Q N Xu, and
B Li
December 1987,
Hinyokika kiyo. Acta urologica Japonica,
Q Huang, and
Z W Du, and
G D Xu, and
Z Y Liu, and
Y H Guo, and
G L Chen, and
W X Ma, and
Q Y Tan, and
Q N Xu, and
B Li
September 1985,
Journal of neuropathology and experimental neurology,
Q Huang, and
Z W Du, and
G D Xu, and
Z Y Liu, and
Y H Guo, and
G L Chen, and
W X Ma, and
Q Y Tan, and
Q N Xu, and
B Li
December 1981,
Nihon Hinyokika Gakkai zasshi. The japanese journal of urology,
Q Huang, and
Z W Du, and
G D Xu, and
Z Y Liu, and
Y H Guo, and
G L Chen, and
W X Ma, and
Q Y Tan, and
Q N Xu, and
B Li
February 2015,
Zhonghua zhong liu za zhi [Chinese journal of oncology],
Q Huang, and
Z W Du, and
G D Xu, and
Z Y Liu, and
Y H Guo, and
G L Chen, and
W X Ma, and
Q Y Tan, and
Q N Xu, and
B Li
July 1984,
Zhonghua zhong liu za zhi [Chinese journal of oncology],
Q Huang, and
Z W Du, and
G D Xu, and
Z Y Liu, and
Y H Guo, and
G L Chen, and
W X Ma, and
Q Y Tan, and
Q N Xu, and
B Li
October 1987,
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae,
Q Huang, and
Z W Du, and
G D Xu, and
Z Y Liu, and
Y H Guo, and
G L Chen, and
W X Ma, and
Q Y Tan, and
Q N Xu, and
B Li
March 2017,
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology,
Q Huang, and
Z W Du, and
G D Xu, and
Z Y Liu, and
Y H Guo, and
G L Chen, and
W X Ma, and
Q Y Tan, and
Q N Xu, and
B Li
April 1980,
Gan,
Q Huang, and
Z W Du, and
G D Xu, and
Z Y Liu, and
Y H Guo, and
G L Chen, and
W X Ma, and
Q Y Tan, and
Q N Xu, and
B Li
March 1995,
Oncology reports,
