-transparent.png){kind=logo}
A discontinuous Percoll density gradient was employed for the selective isolation of human X-bearing sperm. The fraction rich in X-bearing sperm that sedimented through eight steps of a Percoll density gradient was inseminated into healthy volunteers. Six couples achieved pregnancy and each delivered a female baby without abnormality. For further separation, a Percoll density gradient consisting of 12 steps gave a 94% purification of X-bearing sperm (contamination with Y-bearing sperm: 6.4 +/- 1.8%) with a mean recovery of 23.3 +/- 6.4% (n = 6).
Related Publications
R Iizuka, and
S Kaneko, and
R Aoki, and
T Kobayashi
April 1984,
Medical laboratory sciences,
R Iizuka, and
S Kaneko, and
R Aoki, and
T Kobayashi
January 1996,
Andrologia,
R Iizuka, and
S Kaneko, and
R Aoki, and
T Kobayashi
April 1989,
Fertility and sterility,
R Iizuka, and
S Kaneko, and
R Aoki, and
T Kobayashi
January 1979,
Journal of immunological methods,
R Iizuka, and
S Kaneko, and
R Aoki, and
T Kobayashi
January 1979,
Journal of immunological methods,
R Iizuka, and
S Kaneko, and
R Aoki, and
T Kobayashi
May 1988,
Gamete research,
R Iizuka, and
S Kaneko, and
R Aoki, and
T Kobayashi
July 1982,
Clinica chimica acta; international journal of clinical chemistry,
R Iizuka, and
S Kaneko, and
R Aoki, and
T Kobayashi
August 2011,
In vitro cellular & developmental biology. Animal,
R Iizuka, and
S Kaneko, and
R Aoki, and
T Kobayashi
December 2018,
Animal reproduction science,
R Iizuka, and
S Kaneko, and
R Aoki, and
T Kobayashi
January 1991,
Laboratornoe delo,
