OBJECTIVE The aim of this study was to assess and compare the level of serum vitamin-D in participants affected with recurrent upper respiratory tract infections and in healthy population and to know whether vitamin-D deficiency is factor contributing to recurrent upper respiratory tract infections (URTI). A case control study was conducted on 52 subjects with recurrent URTI and 52 controls. Frequency and severity of infections in the previous 6 months were assessed and documented among the case group. Vitamin D level was assessed in all the participants among case and control group and statistical analysis was done. Mean serum vitamin D was 10.67 ± 3.58 ng/mL in the study group and 20.10 ± 7.73 ng/mL in the control group, the difference in value was statistically significant. None of the study group participants belonged to vitamin D sufficient group, and majority (98%) were in vitamin D deficient, except for 2% who were in insufficient group. In the control group, 10%,29%,61% were in sufficient, insufficient and deficient group respectively. Mean serum vitamin D was not significantly associated with severity and type of infection. Significant number of participants in both study and control had serum vitamin D deficiency. This study also observed that a significant number of participants with recurrent URTI had serum vitamin D deficiency than the control group which suggest that hypovitaminosis D is a factor contributing to recurrent URTI. The online version contains supplementary material available at 10.1007/s12070-022-03220-z.